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Caspase 8 Colorimetric Assay Kit (ab39700)

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    Overview

    Product name

    Caspase 8 Colorimetric Assay Kit
    See all Caspase 8 products (4) ...

    Notes

    The Caspase 8 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD

    The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase 8 activity.

    Properties

    Storage instructions

    Store kit at -20°C (Store Lysis Buffer and 2X Reaction Buffer at +4°C after opening)

    Components 1 kit
    2X Reaction Buffer2 x 4ml
    Cell Lysis Buffer1 x 100ml
    Dilution Buffer1000 x 100ml
    DTT1 x 400µl
    IETD-pNA1 x 500µl

    Research areas

    Kits/ Lysates/ Other >> Kits >> Apoptosis Kits >> Caspase Assays Kits >> Caspase 8 assay kits
    Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Other proteases
    Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
    Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

    Relevance

    Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

    Cellular localization

    Cytoplasmic

    Alternative names

    • Apoptosis related cysteine peptidase
    • Apoptotic cysteine protease
    • Apoptotic protease Mch 5
    • CAP 4
    • CAP4
    • CASP 8
    • CASP8
    • Caspase 8
    • Caspase8
    • CED 3
    • FADD homologous ICE/CED 3 like protease
    • FADD Homologous ICE/CED3 Like Protease
    • FADD Like ICE
    • FLICE
    • ICE like apoptotic protease 5
    • MACH
    • MACH5
    • MCH 5
    • MCH5
    • MGC78473
    • MORT1 associated CED 3 homolog
    • MORT1 associated CED3 homolog
    see all

    Protocols

  • Caspase 8 Colorimetric Assay kit

    • References for Caspase 8 Colorimetric Assay Kit (ab39700)

    ab39700 has not yet been referenced specifically in any publications.

    Publishing research using ab39700? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"