Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)
- Product nameCaspase-8 Human ELISA Kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Serum 6.7% Inter-assay Sample n Mean SD CV% Serum 8.5%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Cell culture extracts, Suspension cells
- Assay typeSandwich
- Sensitivity0.1 ng/ml
- Range0.16 ng/ml - 10 ng/ml
- Species reactivityReacts with: Human
- Product overview
The human Caspase-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-8. The human Caspase-8 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.
Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Anti-human Caspase-8 polyclonal (rabbit) Detection Antibody 1 x 100µl Anti-rabbit-IgG-HRP 1 x 10µl Assay Buffer Concentrate (20x) 1 x 50ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Caspase-8 Standard 2 vials Lysis Buffer (10x) 1 x 15ml Monoclonal anti-human Caspase 8-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
Contains 2 DED (death effector) domains.
modificationsGeneration of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- Apoptotic cysteine proteaseApoptotic protease Mch-5CAP4
- CASP-8CASP8CASP8_HUMANCaspase-8 subunit p10FADD-homologous ICE/CED-3-like proteaseFADD-like ICEFLICEICE-like apoptotic protease 5MACHMORT1-associated CED-3 homolog
Our Abpromise guarantee covers the use of ab119507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Caspase-8 Human ELISA Kit (with color giving dyes) images
Representative standard curve for Human Caspase-8 ELISA. Human Caspase-8 was diluted in serial 2-fold steps in Sample Diluent.
References for Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)
ab119507 has not yet been referenced specifically in any publications.