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Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)

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Overview

  • Product nameCaspase-8 Human ELISA Kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Serum 6.7%
    Inter-assay
    Sample n Mean SD CV%
    Serum 8.5%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts, Suspension cells
  • Assay typeSandwich
  • Sensitivity
    0.1 ng/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Species reactivity
    Reacts with: Human
  • Product overview

    The human Caspase-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-8. The human Caspase-8 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

    Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.

    Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    Adhesive Films 4 units
    Anti-human Caspase-8 polyclonal (rabbit) Detection Antibody 1 x 100µl
    Anti-rabbit-IgG-HRP 1 x 10µl
    Assay Buffer Concentrate (20x) 1 x 50ml
    Blue-Dye 1 x 0.4ml
    Green-Dye 1 x 0.4ml
    Human Caspase-8 Standard 2 vials
    Lysis Buffer (10x) 1 x 15ml
    Monoclonal anti-human Caspase 8-coated microwell plate 1 unit
    Red-Dye 1 x 0.4ml
    Sample Diluent 1 x 12ml
    Stop Solution 1 x 15ml
    Substrate Solution 1 x 15ml
    Wash Buffer Concentrate (20x) 1 x 50ml
  • Research Areas
  • FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Apoptotic cysteine proteaseApoptotic protease Mch-5CAP4
        CASP-8CASP8CASP8_HUMANCaspase-8 subunit p10FADD-homologous ICE/CED-3-like proteaseFADD-like ICEFLICEICE-like apoptotic protease 5MACHMORT1-associated CED-3 homolog
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119507 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Caspase-8 Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)
        Sandwich ELISA - Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)
        Representative standard curve for Human Caspase-8 ELISA. Human Caspase-8 was diluted in serial 2-fold steps in Sample Diluent.

      Protocols

      References for Caspase-8 Human ELISA Kit (with color giving dyes) (ab119507)

      ab119507 has not yet been referenced specifically in any publications.

      Publishing research using ab119507 ? Please let us know so that we can cite the reference in this datasheet

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      I lysed my cells few weeks ago using the lysis buffer that was supplied with [ab119507 Caspase-8 Human ELISA Kit]. I would like to run another ELISA experiment to detect cytochrome C release from the mitochondria, can I use the cell lysate that I have fr...

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      The lysis buffer in kits ab119507 and ab119521 are identical, so you can analyze lysates prepared for ab119507 with ab119521.
      For ab119521, the lysis buffer allows for lysis of the cellular membrane but not other compartments with in the cy...

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      I am going to lyse my cells using the lysis buffer that is supplied with the csapase-8 kit and run the caspase-8 test.also I will save some lysate for later experiments.

      my question is: can I use this lysate to run the caspase-9 test when I rece...

      Read More


      The lysis buffers in both kits (ab119507 and ab119508) are the same and thus interchangeable.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"