For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome.
Tanzania, United Republic of
Antigua and Barbuda
Saint Kitts and Nevis
Saint Pierre and Miquelon
Trinidad & Tob
Korea, Rep of
Papua New Guinea
Bosnia and Herzegovina
Abcam's Caspase 8 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate IETD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 8 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 8 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
|Active Caspase 8||1 x 100 units|
|Caspase Inhibitor, Z-VAD-FMK||1 x 10µl|
|Caspase Substrate IETD-AFC||1 x 0.5ml|
|DTT||1 x 100µl|
|Reaction buffer||1 x 10ml|
Our Abpromise guarantee covers the use of ab102496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using IETD-AFC as caspase 8 substrate and following Caspase 8 Inhibitor Drug Detection Kit (ab102496) protocol. Lanes:
1.- Background Control: no Caspase 8, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 8, +z-VAD-FMK
3.- Positive Control: + Caspase 8, no z-VAD-FMK
4.- Caspase 8 + 2.5µM z-VAD-FMK
5.- Caspase 8 + 10µM z-VAD-FMK
6.- Caspase 8 + 40µM z-VAD-FMK
ab102496 has not yet been referenced specifically in any publications.
Publishing research using ab102496 ? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"