Overview
- Product nameCaspase 8 Inhibitor Drug Detection Kit
- Tests100 x 1 assay
- Sample typeAdherent cells, Suspension cells
- Assay typeQuantitative
- Assay time1h 30m
- Product overview
Abcam's Caspase 8 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate IETD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 8 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 8 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. - Notes
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
- Tested applicationsFunctional Studies more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components 100 tests Active Caspase 8 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate IETD-AFC 1 x 0.5ml DTT 1 x 100µl Reaction buffer 1 x 10ml - Research Areas
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Alternative names
- CASP8 inhibitor
Applications
Our Abpromise guarantee covers the use of ab102496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Protocols
References for Caspase 8 Inhibitor Drug Detection Kit (ab102496)
ab102496 has not yet been referenced specifically in any publications.
