Anti-Caspase-8 antibody [ABM14C1] (ab220171)
Key features and details
- Mouse monoclonal [ABM14C1] to Caspase-8
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Caspase-8 antibody [ABM14C1]
See all Caspase-8 primary antibodies -
Description
Mouse monoclonal [ABM14C1] to Caspase-8 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Caspase-8 aa 150-400.
Database link: Q14790 -
Positive control
- WB: Molt-4, Kato III and HepG2 cell lysate. IHC-P: Human esophagus and tonsil tissue. Flow Cyt: HeLa and Jurkat cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
ABM14C1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 55 kDa.
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Flow Cyt |
Use 0.5-1µg for 106 cells.
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IHC-P |
Use a concentration of 5 - 10 µg/ml.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 55 kDa. |
Flow Cyt
Use 0.5-1µg for 106 cells. |
IHC-P
Use a concentration of 5 - 10 µg/ml. |
Target
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Function
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
Tissue specificity
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. -
Involvement in disease
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. -
Sequence similarities
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
Domain
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. -
Post-translational
modificationsGeneration of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 841 Human
- Omim: 601763 Human
- SwissProt: Q14790 Human
- Unigene: 599762 Human
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Alternative names
- ALPS2B antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
- Apoptosis related cysteine peptidase antibody
see all
Images
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Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human esophagus tissue sections labeling Caspase-8 with ab220171 at 5 µg/mL.
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All lanes : Anti-Caspase-8 antibody [ABM14C1] (ab220171) at 2 µg/ml
Lane 1 : Molt-4 cell lysate
Lane 2 : Kato III cell lysate
Lane 3 : HepG2 cell lysate
Predicted band size: 55 kDa -
Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue sections labeling Caspase-8 with ab220171 at 5 µg/mL.
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Flow Cytometry analysis of HeLa cells labeling Caspase-8 with ab220171 at 0.5 µg/106 cells.
ab220171: red
Isotype control: green
A goat anti-mouse PE conjugate was used as the secondary antibody.
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Flow Cytometry analysis of Jurkat cells lableing Caspase-8 with ab220171 at 0.5 µg/106 cells.
ab220171: red
Isotype control: green
A goat anti-mouse PE conjugate was used as the secondary antibody.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab220171 has been referenced in 2 publications.
- Yao B et al. High-matrix-stiffness induces promotion of hepatocellular carcinoma proliferation and suppression of apoptosis via miR-3682-3p-PHLDA1-FAS pathway. J Cancer 11:6188-6203 (2020). PubMed: 33033502
- Lin MX et al. Lobocrassin B Induces Apoptosis of Human Lung Cancer and Inhibits Tumor Xenograft Growth. Mar Drugs 15:N/A (2017). PubMed: 29207557