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Anti-Caspase 8 antibody [C502S] (ab2553)

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Overview

  • Product nameAnti-Caspase 8 antibody [C502S]See all Caspase 8 primary antibodies ...
  • Description
    Mouse monoclonal [C502S] to Caspase 8
  • SpecificityThis antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8.
  • Tested applicationsIP, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Available on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab2553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IP IP: Use a concentration of 10 µg/ml.
WB WB: Use a concentration of 1 µg/ml.

Target

  • FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        ALPS2B antibodyAmyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibodyApoptosis related cysteine peptidase antibody
        Apoptotic cysteine protease antibodyApoptotic protease Mch 5 antibodyApoptotic protease Mch-5 antibodyApoptotic protease Mch5 antibodyCAP 4 antibodyCAP4 antibodyCASP 8 antibodyCASP-8 antibodyCASP8 antibodyCASP8_HUMAN antibodyCaspase 8 antibodyCaspase 8 apoptosis related cysteine peptidase antibodyCaspase-8 subunit p10 antibodyCaspase8 antibodyCED 3 antibodyFADD homologous ICE/CED 3 like protease antibodyFADD Homologous ICE/CED3 Like Protease antibodyFADD Like ICE antibodyFADD-homologous ICE/CED-3-like protease antibodyFADD-like ICE antibodyFLICE antibodyFLJ17672 antibodyICE like apoptotic protease 5 antibodyICE-like apoptotic protease 5 antibodyMACH alpha 1/2/3 protein antibodyMACH antibodyMACH beta 1/2/3/4 protein antibodyMCH 5 antibodyMCH5 antibodyMGC78473 antibodyMORT1 associated CED 3 homolog antibodyMORT1 associated CED3 homolog antibodyMORT1-associated CED-3 homolog antibodyOTTHUMP00000163717 antibodyOTTHUMP00000163720 antibodyOTTHUMP00000163724 antibodyOTTHUMP00000163725 antibodyOTTHUMP00000165062 antibodyOTTHUMP00000165063 antibodyOTTHUMP00000165064 antibodyOTTHUMP00000206552 antibodyOTTHUMP00000206582 antibody
      see all

    References for Anti-Caspase 8 antibody [C502S] (ab2553)

    ab2553 has not yet been referenced specifically in any publications.

    Publishing research using ab2553 ? Please let us know so that we can cite the reference in this datasheet

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    Thank you for your explanation. In the case of using a mouse Ab in IHC for mouse tissues, one can label the primary Ab with Biotin to avoid erroneous results. Your antibody is the only one I can found that only recognize cleaved caspase-8 fragment. So do ...

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    Thank you for your message. As it has not been tested yet with mouse tissue, we cannot provide any guarantees, but we would be very interested to hear how it works out for you if you do decide to try it. If you have any more questions, please do contact...

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    Hi, From reading some of the earlier enquiries, I understand that this caspase-8 Ab (C502S) only recongnizes human p10 but not the proform of caspase-8. I am looking for a caspase-8 antibody that only recongnizes activated caspase-8 in tissue section by I...

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    Ab2553 has only been tested so far for cross-reactivity with human and tested in Western blotting and IP. I would not recommend this antibody to try with mouse samples in IHC - For immunohistochemistry, the primary antibody should be raised in a species...

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    Do these antibodies recognize both the active and the inactive forms of Caspase or only the active form? Could you please confirm it?

    Thank you for your enquiry. This antibody recognizes only the active form.

    I want to confirm that the sequence I am using is actually from human and not mouse caspase as you seem to think, in which case would you mind double checking that our sequences are not the same? Thanks

    The information I have on file and can release is that the immunogen is a 12 a.a peptide corresponding to the N-terminus of the p10 subunit of human caspase-8. I cannot release the exact sequence, but if customer is working on caspases, he or she can easi...

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    Thank you for your previous information on this enquiry. The customer understands that you m,ay not wish to reveal your sequence used but wondered if you tell her if her sequence (LSSPQTRYIPDE) is not the same. thanks

    The customer's sequence seems to be derived from mouse caspase-8. ab2553 utilizes the sequence derived from human. There are a few amino acids are different between human and mouse caspase-8.

    Thank you very much for the information. I have just placed an order for this Ab via my accounts department. Please could you tell me the sequence of the immunogen used to produce the Ab.

    The immunogen is the synthetic peptide corresponding to residues mapping at the N-terminus of the p10 fragment of caspase-8. Gloria

    Caspase 8 antibody (ab2553) clone C502S. Does this Ab detect the p10 only or does it also detect the proform and the other cleaved fragments? I am interested in an Ab which only detects p10 .

    Yes, the antibody only detects p10 fragment of the cleaved caspase-8.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"