Anti-Caspase 8 antibody [E7] (ab32397)

Overview

• Product nameAnti-Caspase 8 antibody [E7]See all Caspase 8 primary antibodies ...
• Description
  Rabbit monoclonal [E7] to Caspase 8
• SpecificityThe antibody should recognize both pro-form (55kDa) and p18 cleaved-form of Caspase-8.
• Tested applicationsWB, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to N-terminal residues of human Caspase-8 subunit p18 was used as immunogen.

• Positive controlHela cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberE7
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Caspases

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Other proteases

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Caspases

Applications

Target

• FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
  -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
• Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
• Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
• Sequence similaritiesBelongs to the peptidase C14A family.
  Contains 2 DED (death effector) domains.
• DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
• Post-translational
  modificationsGeneration of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
  Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q14790 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 841 Human
  • Omim: 601763 Human
  • SwissProt: Q14790 Human
  • Unigene: 655983 Human
  • Unigene: 599762 Human

• Alternative names
  ALPS2B antibodyAmyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibodyApoptosis related cysteine peptidase antibody

  Apoptotic cysteine protease antibodyApoptotic protease Mch 5 antibodyApoptotic protease Mch-5 antibodyApoptotic protease Mch5 antibodyCAP 4 antibodyCAP4 antibodyCASP 8 antibodyCASP-8 antibodyCASP8 antibodyCASP8_HUMAN antibodyCaspase 8 antibodyCaspase 8 apoptosis related cysteine peptidase antibodyCaspase-8 subunit p10 antibodyCaspase8 antibodyCED 3 antibodyFADD homologous ICE/CED 3 like protease antibodyFADD Homologous ICE/CED3 Like Protease antibodyFADD Like ICE antibodyFADD-homologous ICE/CED-3-like protease antibodyFADD-like ICE antibodyFLICE antibodyFLJ17672 antibodyICE like apoptotic protease 5 antibodyICE-like apoptotic protease 5 antibodyMACH alpha 1/2/3 protein antibodyMACH antibodyMACH beta 1/2/3/4 protein antibodyMCH 5 antibodyMCH5 antibodyMGC78473 antibodyMORT1 associated CED 3 homolog antibodyMORT1 associated CED3 homolog antibodyMORT1-associated CED-3 homolog antibodyOTTHUMP00000163717 antibodyOTTHUMP00000163720 antibodyOTTHUMP00000163724 antibodyOTTHUMP00000163725 antibodyOTTHUMP00000165062 antibodyOTTHUMP00000165063 antibodyOTTHUMP00000165064 antibodyOTTHUMP00000206552 antibodyOTTHUMP00000206582 antibody

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Anti-Caspase 8 antibody [E7] images

• 

  Western blot - Caspase 8 antibody [E7] (ab32397)
  Anti-Caspase 8 antibody [E7] (ab32397) at 1/500 dilution + 10ug HeLa cell lysate.
  
  Predicted band size : 55 kDa
  Observed band size : 55 kDa