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Overview

  • Product nameCaspase 8 protein (Active)See all Caspase 8 proteins and peptides ...
  • Protein descriptionRecombinant full length Caspase 8 protein (Human)
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activitySpecific Activity: 5000 units/mg. One unit of the recombinant Caspase 8 is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab54117 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    Functional Studies FuncS: Use at an assay dependent concentration. Caspase Activity: Use 1 unit/assay.
    WB WB: Use at an assay dependent concentration. Ab54117 can be used as a WB positive control in conjunction with ab25901.

    Protein info

    • Alternative names
        ALPS2BAmyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 proteinApoptosis related cysteine peptidase
        Apoptotic cysteine proteaseApoptotic protease Mch 5Apoptotic protease Mch-5Apoptotic protease Mch5CAP 4CAP4CASP 8CASP-8CASP8CASP8_HUMANCaspase 8Caspase 8 apoptosis related cysteine peptidaseCaspase-8 subunit p10Caspase8CED 3FADD homologous ICE/CED 3 like proteaseFADD Homologous ICE/CED3 Like ProteaseFADD Like ICEFADD-homologous ICE/CED-3-like proteaseFADD-like ICEFLICEFLJ17672ICE like apoptotic protease 5ICE-like apoptotic protease 5MACHMACH alpha 1/2/3 proteinMACH beta 1/2/3/4 proteinMCH 5MCH5MGC78473MORT1 associated CED 3 homologMORT1 associated CED3 homologMORT1-associated CED-3 homologOTTHUMP00000163717OTTHUMP00000163720OTTHUMP00000163724OTTHUMP00000163725OTTHUMP00000165062OTTHUMP00000165063OTTHUMP00000165064OTTHUMP00000206552OTTHUMP00000206582
      see all
  • FunctionMost upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • Tissue specificityIsoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • Involvement in diseaseDefects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • DomainIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • Post-translational
    modifications    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Caspase 8 protein (Active) images

    • Western blot
      Western blot
      Anti-Caspase 8 antibody (ab25901) at 1 µg/ml + Caspase 8 protein (Active) (ab54117) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 1 minute

    References for Caspase 8 protein (Active) (ab54117)

    ab54117 has not yet been referenced specifically in any publications.

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