You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Caspase-9 Human ELISA Kit (with color giving dyes) (ab119508)

Submit an Abreview Q&A (1)

Overview

  • Product nameCaspase-9 Human ELISA Kit (with color giving dyes)
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 6.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Tests
    1 x 96 test
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate
  • Assay typeSandwich
  • Sensitivity
    0.4 ng/ml
  • Range
    1.6 ng/ml - 100 ng/ml
  • Recovery

    103 %

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab119508 Caspase-9 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-9. ab119508 is for research use only. Not for diagnostic or therapeutic procedures.

    Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for Aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.

    Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by Cytochrome C and dATP.

    Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its sub-cellular redistribution and activation. Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and 7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade.

    Feedback cleavage of Caspase-9 by Caspase-3 again significantly increases the activity of the apoptosome. Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimers disease, Huntingtons disease and osteoarthritis.

    In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

    • Target information above from: UniProt accession P55211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        APAF-3APAF3Apoptotic protease Mch-6
        Apoptotic protease-activating factor 3CASP-9CASP9CASP9_HUMANCaspase 9 apoptosis related cysteine peptidaseCaspase-9 subunit p10ICE-LAP6ICE-like apoptotic protease 6MCH6PPP1R56Protein phosphatase 1 regulatory subunit 56
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab119508 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Caspase-9 Human ELISA Kit (with color giving dyes) images

      • Sandwich ELISA - Caspase -9 Human ELISA Kit (with color giving dyes) (ab119508)
        Sandwich ELISA - Caspase -9 Human ELISA Kit (with color giving dyes) (ab119508)
        Representative standard curve for Human Caspase-9 ELISA. Human Caspase-9 was diluted in serial 2-fold steps in Sample Diluent.

      Protocols

      References for Caspase-9 Human ELISA Kit (with color giving dyes) (ab119508)

      ab119508 has not yet been referenced specifically in any publications.

      Publishing research using ab119508 ? Please let us know so that we can cite the reference in this datasheet

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"