Caspase-9 Human ELISA Kit (with color giving dyes) (ab119508)
- Product nameCaspase-9 Human ELISA Kit (with color giving dyes)
Intra-assay Sample n Mean SD CV% Overall 6.6% Inter-assay Sample n Mean SD CV% Overall 9%
- Tests1 x 96 test
- Sample typeCell culture supernatant, Serum, Cell Lysate
- Assay typeSandwich
- Sensitivity0.4 ng/ml
- Range1.6 ng/ml - 100 ng/ml
- Species reactivityReacts with: Human
- Product overview
ab119508 Caspase-9 Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-9. ab119508 is for research use only. Not for diagnostic or therapeutic procedures.
Caspases are the executioners of apoptosis. This cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for Aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis.
Caspase-9, formerly called Apaf-3, binds to Apaf-1 in the presence of cytochrome c and dATP (3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3; caspase-9 thus is the most upstream member of the apoptotic protease cascade that is triggered by Cytochrome C and dATP.
Caspase-9 is essentially localized in mitochondria, the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its sub-cellular redistribution and activation. Apart from cleavage of caspase-3, the downstream enzymes caspase-6 and 7 are targets of caspase-9 activity. Caspase-9 forms dimers in the course of activation. Cytochrome c/Apaf-1/caspase-9 form a so called apoptosome, amplifying the caspase cascade.
Feedback cleavage of Caspase-9 by Caspase-3 again significantly increases the activity of the apoptosome. Caspase-9 plays a crucial role in all pathologies involving apoptotic processes. Specifically the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimers disease, Huntingtons disease and osteoarthritis.
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests Adhesive Films 4 units Anti-human Caspase-9 polyclonal Detection Antibody 1 x 100µl Anti-rabbit-IgG-HRP 1 x 10µl Assay Buffer Concentrate (20x) 1 x 50ml Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Caspase-9 standard 2 vials Lysis buffer (10x) 1 x 15ml Monoclonal anti-human Caspase 9-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml
- Research Areas
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Contains 1 CARD domain.
modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
- APAF-3APAF3Apoptotic protease Mch-6
- Apoptotic protease-activating factor 3CASP-9CASP9CASP9_HUMANCaspase 9 apoptosis related cysteine peptidaseCaspase-9 subunit p10ICE-LAP6ICE-like apoptotic protease 6MCH6PPP1R56Protein phosphatase 1 regulatory subunit 56
Our Abpromise guarantee covers the use of ab119508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Caspase-9 Human ELISA Kit (with color giving dyes) images
Representative standard curve for Human Caspase-9 ELISA. Human Caspase-9 was diluted in serial 2-fold steps in Sample Diluent.
References for Caspase-9 Human ELISA Kit (with color giving dyes) (ab119508)
ab119508 has not yet been referenced specifically in any publications.