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Product name
Anti-Caspase 9 antibody [1-2]
See all Caspase 9 products (27) ...
Description
Mouse monoclonal [1-2] to Caspase 9
Specificity
This antibody reacts with the zymogen form of caspase-9 (46-48 kDa).
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment (Human).
Epitope
This epitope is found at the NH terminus of caspase-9.
Positive control
HeLa or Jurkat cells
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.2% Gelatin, 0.05M Sodium phosphate
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
1-2
Isotype
IgG1
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 46 kDa.
Application notes
Is unsuitable for ,IF,IHC-Fr,IHC-P or IP.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 3 antibody
- APAF-3 antibody
- APAF3 antibody
see all
Database links
- Entrez Gene: 842 Human
- Omim: 602234 Human
- SwissProt: P55211 Human
- Unigene: 329502 Human
References for Anti-Caspase 9 antibody [1-2] (ab16893)
ab16893 has not yet been referenced specifically in any publications.
Publishing research using ab16893? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"