Caspase 9 antibody [5B4] (ab28131)

Product Name 

Caspase 9 antibody [5B4]

Product type 

Primary antibodies

Description 

Mouse monoclonal [5B4] to Caspase 9

Immunogen 

Human Caspase 9 fusion protein fragment, corresponding to N terminal amino acids 1 - 250 of Human Caspase 9.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Ab28131 is specific to Caspase 9.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Bands of approximately 46 kDa (procaspase 9) and/or 37 kDa (cleaved caspase 9) and/or 35 kDa (cleaved caspase 9) may be observed (predicted molecular weight: 46 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa and Jurkat cell lysates.

Cellular localization 

Cytoplasmic and Mitochondrial

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

Relevance 

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

5B4

Isotype 

IgG1

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2.

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

References for Caspase 9 antibody [5B4] (ab28131)

  This clone has been used in:

Yakovlev AG et al. Differential expression of apoptotic protease-activating factor-1 and caspase-3 genes and susceptibility to apoptosis during brain development and after traumatic brain injury. J Neurosci 21:7439-46 (2001). PubMed: 11567033
If you publish research using ab28131 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Caspase 9 antibody [5B4]

Customer FAQs regarding Caspase 9 antibody [5B4]

Protocols for Caspase 9 antibody [5B4]

Price and availability of products related to Caspase 9 antibody [5B4]

Caspase 9 antibody [5B4] - related products: