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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Overview
Product name
Anti-Caspase 9 antibody [5B4]
See all Caspase 9 products (27) ...
Description
Mouse monoclonal [5B4] to Caspase 9
Specificity
Ab28131 is specific to Caspase 9.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Human Caspase 9 fusion protein fragment, corresponding to N terminal amino acids 1 - 250 of Human Caspase 9.
Positive control
HeLa and Jurkat cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS, pH 7.2.
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
5B4
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at a concentration of 1 µg/ml. Bands of approximately 46 kDa (procaspase 9) and/or 37 kDa (cleaved caspase 9) and/or 35 kDa (cleaved caspase 9) may be observed (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 3 antibody
- APAF-3 antibody
- APAF3 antibody
see all
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q4FJK5 Mouse
- SwissProt: Q920G4 Rat
- Unigene: 329502 Human
see all
References for Anti-Caspase 9 antibody [5B4] (ab28131)
This product has been referenced in:
- Akpan Net al. Intranasal delivery of caspase-9 inhibitor reduces caspase-6-dependent axon/neuron loss and improves neurological function after stroke. J Neurosci 31:8894-904 (2011). WB, IHC-Fr; Rat.Read more (PubMed: 21677173) »
- Gullberg Met al. A single coxsackievirus B2 capsid residue controls cytolysis and apoptosis in rhabdomyosarcoma cells. J Virol 84:5868-79 (2010). WB; Human.Read more (PubMed: 20375176) »
See all 3 publications for this product
Publishing research using ab28131? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"