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Synthetic peptide corresponding to Human Caspase-9 aa 304-315. Synthetic peptide:
CEDESPGSNPEPD, corresponding to amino acids 304-315 of Human Caspase 9 (UniProt ID: P55211).
The ab133981 target is the carboxy terminal end of the larger subunit of Caspase-9 (AA? – AA315) that can exist as a 35 kDa Caspase-9 fragment (p35, AA1-AA315) or as a smaller further cleaved fragment of 17-25 kDa (AA?-AA315).
Our Abpromise guarantee covers the use of ab133981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 35 kDa.|
Western Blot using mouse Cleaved (Asp315) Caspase-9 antibody D10CG8 (ab133981) and rabbit polyclonal Caspase-9 antibody (ab25758) with infra-red detection.
A: an image of anti-Cleaved (Asp315) Caspase-9 antibody D10CG8 (ab133981) signal detected with goat anti-mouse-secondary antibody is shown in green.
B: an image of anti- Caspase-9 antibody (ab25758) signal detected with goat anti rabbit-secondary antibody is shown in red.
C: an overlay of images shown in A and B. Note the perfect overlay (in yellow) of the 20 kDa band in lane 2 and of the 23 kDa band in lane 5. Note also the specificity of the Cleaved (Asp315) Caspase 9 antibody towards the recombinant Caspase 9, but not towards the recombinant Caspase 3.
Lane 1: Marker
Lane 2: Human recombinant active Caspase 9 protein (ab52203), 20 ng
Lane 3: Human recombinant active Caspase 3 protein (ab52314), 20 ng
Lane 4: RIPA extract of HeLa cells treated with vehicle (DMSO)
ab133981 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"