MitoSciences (MS1105)

Anti-Caspase-9 antibody [D10CG8] (ab133981)

Overview

  • Product nameAnti-Caspase-9 antibody [D10CG8]
    See all Caspase-9 primary antibodies
  • Description
    Mouse monoclonal [D10CG8] to Caspase-9
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 304-315. Synthetic peptide:

    CEDESPGSNPEPD

    , corresponding to amino acids 304-315 of Human Caspase 9 (UniProt ID: P55211).
    Sequence:

    CEDESPGSNPEPD


    Database link: P55211

  • Positive control
    • Human recombinant active Caspase-9 protein; RIPA extract of HeLa cells treated for 4 hours with 1 µM Staurosporine
  • General notes

     

    The ab133981 target is the carboxy terminal end of the larger subunit of Caspase-9 (AA? – AA315) that can exist as a 35 kDa Caspase-9 fragment (p35, AA1-AA315) or as a smaller further cleaved fragment of 17-25 kDa (AA?-AA315).

Properties

Applications

Our Abpromise guarantee covers the use of ab133981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 35 kDa.
  • Application notesIs unsuitable for ICC or IP.
  • Target

    • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
      Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
    • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
    • Sequence similaritiesBelongs to the peptidase C14A family.
      Contains 1 CARD domain.
    • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
    • Post-translational
      modifications
      Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
    • Information by UniProt
    • Database links
    • Alternative names
      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    Anti-Caspase-9 antibody [D10CG8] images

    • All lanes : Anti-Caspase-9 antibody [D10CG8] (ab133981) at 2 µg/ml

      Lane 1 : Human recombinant active Caspase 9 protein at 0.02 µg
      Lane 2 : RIPA extract of HeLa cells treated with vehicle (DMSO) at 40 µg
      Lane 3 : RIPA extract of HeLa cells treated with 1 uM staurosporine at 40 µg

      Secondary
      Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/5000 dilution
      developed using the ECL technique

      Predicted band size : 35 kDa
      Observed band size : 20,23,37 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 55 kDa (possible cross reactivity).

      Exposure time : 30 seconds


    • Predicted band size : 35 kDa

      Western Blot using mouse Cleaved (Asp315) Caspase-9 antibody D10CG8 (ab133981) and rabbit polyclonal Caspase-9 antibody (ab25758) with infra-red detection.
      A: an image of anti-Cleaved (Asp315) Caspase-9 antibody D10CG8 (ab133981) signal detected with goat anti-mouse-secondary antibody is shown in green.
      B: an image of anti- Caspase-9 antibody (ab25758) signal detected with goat anti rabbit-secondary antibody is shown in red.
      C: an overlay of images shown in A and B. Note the perfect overlay (in yellow) of the 20 kDa band in lane 2 and of the 23 kDa band in lane 5. Note also the specificity of the Cleaved (Asp315) Caspase 9 antibody towards the recombinant Caspase 9, but not towards the recombinant Caspase 3.

      Lane 1: Marker
      Lane 2: Human recombinant active Caspase 9 protein (ab52203), 20 ng
      Lane 3: Human recombinant active Caspase 3 protein (ab52314), 20 ng
      Lane 4: RIPA extract of HeLa cells treated with vehicle (DMSO)

    References for Anti-Caspase-9 antibody [D10CG8] (ab133981)

    ab133981 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"