Anti-Caspase 9 antibody [D10CG8] (ab133981)

Overview

• Product nameAnti-Caspase 9 antibody [D10CG8]See all Caspase 9 primary antibodies ...
• Description
  Mouse monoclonal [D10CG8] to Caspase 9
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide: CEDESPGSNPEPD, corresponding to amino acids 304-315 of Human Caspase 9 (UniProt ID: P55211).

  Run BLAST with Run BLAST with
• Positive controlHuman recombinant active Caspase 9 protein; RIPA extract of HeLa cells treated for 4 hours with 1 µM Staurosporine

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: 99% HBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesNear homogeneity, as judged by SDS-PAGE. ab133981 was produced in vitro using hybridomas grown in serum free medium, and then purified by biochemical fractionation.
• Primary antibody notes The ab133981 target is the carboxy terminal end of the larger subunit of Caspase 9 (AA? – AA315) that can exist as a 35 kDa Caspase 9 fragment (p35, AA1-AA315) or as a smaller further cleaved fragment of 17-25 kDa (AA?-AA315).
• Clonality Monoclonal
• Clone numberD10CG8
• IsotypeIgG2b
• Light chain typekappa
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Caspases

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Cytochrome C

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Caspases etc
  • Caspases

Applications

Target

• FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
  Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
• Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
• Sequence similaritiesBelongs to the peptidase C14A family.
  Contains 1 CARD domain.
• Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
• Post-translational
  modificationsCleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

Target information above from: UniProt accession P55211 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 842 Human
  • Omim: 602234 Human
  • SwissProt: P55211 Human
  • Unigene: 329502 Human

• Alternative names
  APAF-3 antibodyAPAF3 antibodyApoptosis related cysteine peptidase antibody

  Apoptotic protease Mch-6 antibodyApoptotic protease-activating factor 3 antibodyCASP-9 antibodyCASP9 antibodyCASP9_HUMAN antibodyCaspase 9 apoptosis related cysteine peptidase antibodyCaspase 9 Dominant Negative antibodyCaspase 9c antibodyCaspase-9 antibodyCaspase-9 subunit p10 antibodyICE LAP6 antibodyICE like apoptotic protease 6 antibodyICE-LAP6 antibodyICE-like apoptotic protease 6 antibodyMCH6 antibodyRNCASP9 antibody

  see all

Anti-Caspase 9 antibody [D10CG8] images

• 

  Western blot - Anti-Caspase 9 antibody [D10CG8] (ab133981)
  All lanes : Anti-Caspase 9 antibody [D10CG8] (ab133981) at 2 µg/ml
  
  Lane 1 : Human recombinant active Caspase 9 protein at 0.02 µg
  Lane 2 : RIPA extract of HeLa cells treated with vehicle (DMSO) at 40 µg
  Lane 3 : RIPA extract of HeLa cells treated with 1 uM staurosporine at 40 µg
  
  Secondary
  Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1/5000 dilution
  developed using the ECL technique
  
  Predicted band size : 35 kDa
  Observed band size : 20,23,37 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 55 kDa (possible cross reactivity).
  
  Exposure time : 30 seconds
• 

  Western blot - Anti-Caspase 9 antibody [D10CG8] (ab133981)
  
  
  Predicted band size : 35 kDa