Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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ab32539 has been referenced in 5 publications.
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All lanes : Anti-Caspase 9 antibody [E23] (ab32539)
Lane 1 : Jurkat cell lysate -untreated
Lane 2 : Jurkat cell lysate - treated with Camptothecin
Predicted band size : 46 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.
Dilution used: 1/20,000
Overlay histogram showing K562 cells stained with ab32539 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
ab32539 staining Caspase 9 in rat brain tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde, blocked using 1.5% serum for 1 hour at 22°C and then incubated with ab32539 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 594 conjugated goat polyclonal used at a 1/250.
Image courtesy of Dr Nitsan Kozlovsky by Abreview.
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