Caspase 9 antibody [E23] (ab32539)

Product Name 

Caspase 9 antibody [E23]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [E23] to Caspase 9

Immunogen 

Synthetic peptide (Human) - corresponding to the C terminus of Caspase 9 p35 subunit.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody should recognise both the pro-[40kDa] form and p35 cleaved form of Caspase 9.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/100.
ICC: 1/50.
IHC-P: 1/50 - 1/100.
IP: 1/100.
WB: Use at an assay dependent dilution. Predicted molecular weight: 46 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cell lysate, human cervical carcinoma.

Cellular localization 

Cytoplasmic and Mitochondrial

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

Relevance 

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

E23

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for Caspase 9 antibody [E23] images, references, products related to ab32539 and other tools.

Caspase 9 antibody [E23] images:

References for Caspase 9 antibody [E23] (ab32539)

  This product has been used in: (two most recent references)

Zhang D et al. Inhibition of pancreatic cancer cell proliferation by propranolol occurs through apoptosis induction: the study of beta-adrenoceptor antagonist's anticancer effect in pancreatic cancer cell. Pancreas 38:94-100 (2009). WB; Human. PubMed: 19106745

Chausiaux OE et al. Hypogonadal mouse, a model to study the effects of the endogenous lack of gonadotropins on apoptosis. Biol Reprod 78:77-90 (2008). PubMed: 17671269
If you publish research using ab32539 please let us know so that we can cite the reference on this datasheet.

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