Anti-Caspase 9 antibody [E23] (ab32539)

  Western blot - Caspase 9 antibody [E23] (ab32539)

All lanes : Anti-Caspase 9 antibody [E23] (ab32539)

Lane 1 : Jurkat cell lysate -untreated
Lane 2 : Jurkat cell lysate - treated with Camptothecin


Predicted band size : 46 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

Dilution used: 1/20,000

  Immunohistochemistry (Paraffin-embedded sections) - Caspase 9 antibody [E23] (ab32539)

  Flow Cytometry-Caspase 9 antibody [E23](ab32539)

Overlay histogram showing K562 cells stained with ab32539 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32539, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.

  Immunohistochemistry (Frozen sections) - Caspase 9 antibody [E23] (ab32539)

ab32539 staining Caspase 9 in rat brain tissue by Immunohistochemistry (Frozen sections).

Tissue was fixed in formaldehyde, blocked using 1.5% serum for 1 hour at 22°C and then incubated with ab32539 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa Fluor 594 conjugated goat polyclonal used at a 1/250.

Image courtesy of Dr Nitsan Kozlovsky by Abreview.

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