Caspase 9 antibody (ab2013)

Product Name 

Caspase 9 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Caspase 9

Immunogen 

Peptide sequence corresponding to amino acids 41 to 56 of human caspase-9.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase 9.

Epitope 

EDIQRAGSGSRRDQAR

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Western blot (1:1000 to 1:2000) gives a band at 46 kD.

Positive control 
(see definition)

HeLa whole cell lysate

Cellular localization 

Cytoplasmic and Mitochondrial

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases

Relevance 

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Caspase 9 is inhibited by the dominant negative isoform, BclXL, cIAP1, cIAP2, XIAP, and Livin. This caspase becomes activated when recruited to Apaf1/cytochrome c complex, and following cleavage by Apaf1, granzyme B, Caspase 3, possibly Caspase 8 and Caspase 10 into large p37 and small p10 subunits. Caspase 9 intereacts with BIRC7 and has been shown to cleave PARP and vimentin.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Caspase 9 antibody images, references, products related to ab2013 and other tools.

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References for Caspase 9 antibody (ab2013)

  This product has been used in:

Jones R Sperm survival versus degradation in the Mammalian epididymis: a hypothesis. Biol Reprod 71:1405-11 (2004). PubMed: 15215193

If you publish research using ab2013 please let us know so that we can cite the reference on this datasheet.

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