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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Overview
Product name
Anti-Caspase 9 antibody
See all Caspase 9 products (27) ...
Description
Rabbit polyclonal to Caspase 9
Specificity
No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase 9.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Peptide sequence corresponding to amino acids 41 to 56 of human caspase-9.
Epitope
EDIQRAGSGSRRDQAR
Positive control
HeLa whole cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Western blot - Caspase 9 antibody (ab2013)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Western blot (1:1000 to 1:2000) gives a band at 46 kD.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 3 antibody
- APAF-3 antibody
- APAF3 antibody
see all
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q4FJK5 Mouse
- SwissProt: Q920G4 Rat
- Unigene: 329502 Human
see all
Anti-Caspase 9 antibody images:
Western blot - Caspase 9 antibody (ab2013)
Anti-Caspase 9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
References for Anti-Caspase 9 antibody (ab2013)
This product has been referenced in:
- Jones R Sperm survival versus degradation in the Mammalian epididymis: a hypothesis. Biol Reprod 71:1405-11 (2004).Read more (PubMed: 15215193) »
See 1 publication for this product
Publishing research using ab2013? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"