Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Caspase 9 antibody
See all Caspase 9 products (27) ...
Description
Rabbit polyclonal to Caspase 9
Specificity
No cross-reactivity to other members of the caspase family.
Tested applications
ICC, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Peptide sequence corresponding to amino acids 299 to 318 ofhuman Caspase 9.
Epitope
ASTSPEDESPGSNPEPDATP
Positive control
HeLa whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC: Use a concentration of 2 µg/ml.
WB: 1/1000 - 1/2000.
IHC-P: Use at an assay dependent dilution.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 3 antibody
- APAF-3 antibody
- APAF3 antibody
see all
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q4FJK5 Mouse
- Unigene: 329502 Human
- Unigene: 88829 Mouse
Anti-Caspase 9 antibody images:
Western blot - Caspase 9 antibody (ab2014)
Anti-Caspase 9 antibody (ab2014) at 1/1000 dilution + HeLa whole cell lysate.
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry - Caspase 9 antibody (ab2014)
ab2014 at 2µg/ml staining Caspase 9 in K562 cells by Immunocytochemistry.
References for Anti-Caspase 9 antibody (ab2014)
This product has been referenced in:
- Minutoli L et al. Mitogen-activated protein kinase 3/mitogen-activated protein kinase 1 activates apoptosis during testicular ischemia-reperfusion injury in a nuclear factor-kappaB-independent manner. Eur J Pharmacol 604:27-35 (2009). WB; Mouse.Read more (PubMed: 19135439) »
- Quintarelli C et al. Co-expression of cytokine and suicide genes to enhance the activity and safety of tumor-specific cytotoxic T lymphocytes. Blood 110:2793-802 (2007). WB.Read more (PubMed: 17638856) »
See all 3 publications for this product
Publishing research using ab2014? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"