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Products:Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Overview
Product name
Anti-Caspase 9 antibody
See all Caspase 9 products (27) ...
Description
Rabbit polyclonal to Caspase 9
Specificity
ab25758 detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved caspase-9. The antibody does not detect any other caspases.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human, Xenopus laevis
Immunogen
Synthetic peptide surrounding amino acid 290 of mouse Caspase 9. (Peptide available as ab38268.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
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Western blot - Caspase 9 antibody (ab25758)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab25758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). Cleaved fragments of 35/37 kDa and 25 kDa are also detected.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- APAF 3 antibody
- APAF-3 antibody
- APAF3 antibody
see all
Database links
- Entrez Gene: 842 Human
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- Omim: 602234 Human
- SwissProt: P55211 Human
- SwissProt: Q4FJK5 Mouse
- SwissProt: Q920G4 Rat
- Unigene: 329502 Human
see all
Anti-Caspase 9 antibody images:
Western blot - Caspase 9 antibody (ab25758)
Anti-Caspase 9 antibody (ab25758) + mouse intestine tissue lysate
Predicted band size : 46 kDa
The antibody detects 45 kDa procaspase 9, 35/37 kDa cleaved immediate fragment and the 17-25 kDa fully cleaved caspase 9.
References for Anti-Caspase 9 antibody (ab25758)
This product has been referenced in:
- Pechenino ASet al. Impact of aging vs. estrogen loss on cardiac gene expression: estrogen replacement and inflammation. Physiol Genomics 43:1065-73 (2011). WB; Rat.Read more (PubMed: 21750230) »
- Stewart ME & Roy P Role of cellular caspases, nuclear factor-kappa B and interferon regulatory factors in Bluetongue virus infection and cell fate. Virol J 7:362 (2010). WB; Human.Read more (PubMed: 21134281) »
See all 4 publications for this product
Publishing research using ab25758? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"