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Product name
Caspase 9 peptide
See all Caspase 9 products (5) ...
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.1% BSA 0.02% sodium azide pH7.2
Concentration
Concentration information loading...
Sequence notes
ASTSPEDESPGSNPEPDATP Amino acids 299 to 318 of human caspase-9 (1-3)
Research areas
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Cancer >> Invasion/microenvironment >> Apoptosis >> Caspases
Cell Biology >> Apoptosis >> Intracellular >> Caspases etc >> Caspases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking
Application notes
The peptide is used for blocking the activity of anti-caspase-9 (ab2013).Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocksthe antibody activity in Western blotting.
Protein info
Function
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Tissue specificity
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Sequence similarities
Belongs to the peptidase C14A family.
Contains 1 CARD domain.
Developmental stage
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
Post-translational
modifications
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
Target information above from: UniProt accessionP55211
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for Caspase 9 peptide (ab7886)
ab7886 has not yet been referenced specifically in any publications.
Publishing research using ab7886? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"