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Overview

  • Product nameCaspase 9 protein (Active)See all Caspase 9 proteins and peptides ...
  • Protein descriptionRecombinant full length Active Caspase 9 protein (Human)
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activitySPECIFIC ACTIVITY: 400 units/mg
  • FormLyophilised:Reconstitute to 1 unit per µl in PBS containing 15% glycerol. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 15% Glycerol, PBS
  • Concentration information loading...
  • Additional notesUNIT DEFINITION: One unit of the recombinant Caspase 9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab52203 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        APAF-3APAF3Apoptosis related cysteine peptidase
        Apoptotic protease Mch-6Apoptotic protease-activating factor 3CASP-9CASP9CASP9_HUMANCaspase 9 apoptosis related cysteine peptidaseCaspase 9 Dominant NegativeCaspase 9cCaspase-9Caspase-9 subunit p10ICE LAP6ICE like apoptotic protease 6ICE-LAP6ICE-like apoptotic protease 6MCH6RNCASP9
      see all
  • FunctionInvolved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
  • Tissue specificityUbiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
  • Sequence similaritiesBelongs to the peptidase C14A family.
    Contains 1 CARD domain.
  • Developmental stageExpressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.
  • Post-translational
    modifications    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.

    • Target information above from: UniProt accession P55211 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for Caspase 9 protein (Active) (ab52203)

    This product has been referenced in:
    • Basu A  et al. Proteolytic activation of protein kinase C-epsilon by caspase-mediated processing and transduction of antiapoptotic signals. J Biol Chem 277:41850-6 (2002). Read more (PubMed: 12198125) »
    • Azuma T  et al. Gelsolin in complex with phosphatidylinositol 4,5-bisphosphate inhibits caspase-3 and -9 to retard apoptotic progression. J Biol Chem 275:3761-6 (2000). Read more (PubMed: 10660524) »

    See all 2 Publications for this product

    Publishing research using ab52203 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab52203 Submit a Question for ab52203

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

    10
    Votes: Highest First

    1 -Is this protein tagged or not? If so, what with?
    2 - What is the amino acid range that the portein covers? Is it the 10 or 12kDa isoform?
    3 - How much (inug) of protein is there per vial? Is it 62.5ug?

    Thank you for contacting Abcam.

    I have spoken to tha lab regarding ab52203 and please find their response below:

    This protein is N-terminal His Tagged.
    The mol wt of this product is ˜30 kDa.
    The amount of protein in terms of &mic...

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    Hi I have a question about ab52203 recombinant full length caspase 9.



    Full length caspase 9 is 46 kDa. Active caspase 9 may be a different molecular weight.



    Western blot and gel analysis of ab52203 show a smal...

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    Thank you for contacting Abcam.

    From the attached data it appears that the antibody 1 and 2 recognize the band which is ˜44 kDa. In addition to the 45 kDa procaspase-9 and 35/37 kDa cleaved large fragment there is also a 25 kDa fully cleaved cas...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"