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ab34157 |
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Read our guarantee »Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Anti-Caspr2 antibody
See all Caspr2 products (5) ...
Rabbit polyclonal to Caspr2
ICC/IF, IHC-FoFr, WBmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Caspr2.
(Peptide available as ab34157.)
This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Rat Brain and Human Brain
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Our Abpromise guarantee covers the use of ab33994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-FoFr: 1/300
WB: Use a concentration of 1 mg/mlDetects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
Predominantly expressed in nervous system.
Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains.
Membrane.
Target information above from: UniProt accessionQ9UHC6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Caspr2 antibody (ab33994)

All lanes : Anti-Caspr2 antibody (ab33994) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate at 20 µg
Lane 2 : Brain (Rat) Tissue Lysate at 20 µg
Lane 3 : Brain (Human) Tissue Lysate at 20 µg
Lane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µg
Lane 5 : Sciatic Nerve (Rat) Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 148 kDa
Observed band size : 152 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)

ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab33994? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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All lanes : Anti-Caspr2 antibody (ab33994) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate at 20 µg
Lane 2 : Brain (Rat) Tissue Lysate at 20 µg
Lane 3 : Brain (Human) Tissue Lysate at 20 µg
Lane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µg
Lane 5 : Sciatic Nerve (Rat) Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 148 kDa
Observed band size : 152 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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