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Products:Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Overview
Product name
Anti-Caspr2 antibody
See all Caspr2 products (5) ...
Description
Rabbit polyclonal to Caspr2
Tested applications
ICC/IF, IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Caspr2.
(Peptide available as ab34157.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Mouse Brain, Rat Brain and Human Brain
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab33994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
IHC-FoFr: 1/300
WB: Use a concentration of 1 mg/mlDetects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).
Target
Function
May play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction.
Tissue specificity
Predominantly expressed in nervous system.
Involvement in disease
Defects in CNTNAP2 are the cause of cortical dysplasia-focal epilepsy syndrome (CDFES) [MIM:610042]. Affected individuals manifest cortical dysplasia, focal epilepsy, relative macrocephaly, and diminished deep-tendon reflexes. Intractable focal seizures begin in early childhood, after which language regression, hyperactivity, impulsive and aggressive behavior, and mental retardation develop.
Genetic variations in CNTNAP2 influences susceptibility to autism type 15 (AUTS15) [MIM:612100]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
Note=A chromosomal aberration involving CNTNAP2 is found in a patient with autism spectrum disorder. Paracentric inversion 46,XY,inv(7)(q11.22;q35). The inversion breakpoints disrupt the genes AUTS2 and CNTNAP2.
Sequence similarities
Belongs to the neurexin family.
Contains 2 EGF-like domains.
Contains 1 F5/8 type C domain.
Contains 1 fibrinogen C-terminal domain.
Contains 4 laminin G-like domains.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9UHC6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell recognition molecule Caspr2 antibody
- Cntnap2 antibody
- CNTP2 antibody
see all
Database links
- Entrez Gene: 26047 Human
- Entrez Gene: 66797 Mouse
- Entrez Gene: 362355 Rat
- Omim: 604569 Human
- SwissProt: Q9UHC6 Human
- SwissProt: Q9CPW0 Mouse
- Unigene: 655684 Human
- Unigene: 440084 Mouse
Anti-Caspr2 antibody images:
Western blot - Caspr2 antibody (ab33994)
All lanes : Anti-Caspr2 antibody (ab33994) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate at 20 µg
Lane 2 : Brain (Rat) Tissue Lysate at 20 µg
Lane 3 : Brain (Human) Tissue Lysate at 20 µg
Lane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µg
Lane 5 : Sciatic Nerve (Rat) Lysate at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 148 kDa
Observed band size : 152 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)
ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-Caspr2 antibody (ab33994)
This product has been referenced in:
- Lai Met al. Investigation of LGI1 as the antigen in limbic encephalitis previously attributed to potassium channels: a case series. Lancet Neurol 9:776-85 (2010). Human.Read more (PubMed: 20580615) »
- Krumbiegel Met al. Genome-wide association study with DNA pooling identifies variants at CNTNAP2 associated with pseudoexfoliation syndrome. Eur J Hum Genet : (2010).Read more (PubMed: 20808326) »
See all 2 publications for this product
Publishing research using ab33994? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"