Feedback Form

Western blot - Caspr2 antibody (ab33994)
Western blot - Caspr2 antibody (ab33994)
(enlarge)


Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)
Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)
(enlarge)


Product Name 

Caspr2 antibody

 

See all Caspr2 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Caspr2

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1300 to the C-terminus of Mouse Caspr2.

(Peptide available as ab34157.)

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

ICC/IF, IHC-FoFr, WB


Abreviews 

Customer reviews
(see key)


Read about Abreviews
(Read all Abreviews)

Submit an Abreview and earn up to 220 Abpoints by reviewing this product

• IHC-FoFr

2 Abreviews

- average rating: 3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)3 stars (out of 5)


IHC-FoFr
(read Abreview)


Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-FoFr: 1/300.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 152 kDa (predicted molecular weight: 148 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following tissue lysates:
Mouse Brain, Rat Brain and Human Brain

Cellular localization 

Cell Membrane

Research areas 

Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker

Relevance 

Caspr2 (for Contactin-associated protein 2)is a mammalian homolog of Drosophila Neurexin IV and belongs to the Neurexin family of highly homologous neuronal cell surface proteins; other members include neurexin I (NRXN1), neurexin II (NRXN2) and neurexin III (NRXN3) and Neurexin IV (Caspr). Caspr2 and the closely related Caspr, demarcate distinct subdomains in myelinated axons. Specifically, Caspr is present at the paranodal junctions, while Caspr2 is colocalized with Shaker-like K+ channels in the juxtaparanodal region.

 

Alternative names Caspr2 antibody (ab33994)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

26047    (Human)
66797    (Mouse)

Omim

    

604569    (Human)

SwissProt

    

Q9UHC6    (Human)
Q9CPW0    (Mouse)
Q3SYI6    (Mouse)

Unigene

    

190621    (Human)
321004    (Mouse)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caspr2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Caspr2 antibody images, references, products related to ab33994 and other tools.

 

Caspr2 antibody images:

Product image  Western blot - Caspr2 antibody (ab33994)

Western blot - Caspr2 antibody (ab33994)

All lanes : Caspr2 antibody (ab33994) at 1 µg/ml

Lane 1 : Brain (Mouse) Tissue Lysate at 20 µg
Lane 2 : Brain (Rat) Tissue Lysate at 20 µg
Lane 3 : Brain (Human) Tissue Lysate at 20 µg
Lane 4 : Sciatic Nerve (Mouse) Lysate at 7.5 µg
Lane 5 : Sciatic Nerve (Rat) Lysate at 20 µg

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 148 kDa
Observed band size : 152 kDa (why is the actual band size different from the predicted?)
Additional bands at : 52 kDa. We are unsure as to the identity of these extra bands.

 

Product image  Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)

Immunocytochemistry/ Immunofluorescence - Caspr2 antibody (ab33994)

ICC/IF image of ab33994 stained SHSY5Y cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33994, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

 
 

Search PubMed (MEDLINE) for references to Caspr2

 

Caspr2 antibody - more information

Customer reviews (feedback) regarding Caspr2 antibody

Customer FAQs regarding Caspr2 antibody

Protocols for Caspr2 antibody

Price and availability of products related to Caspr2 antibody

 

Caspr2 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for Caspr2 antibody

ab34157

  

Caspr2 peptide

 

Compatible Secondaries for Caspr2 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Caspr2 Antibodies

See all Caspr2 antibodies (2)...

 

Caspr2 antibody - other tools:

Contact Abcam with a Technical Enquiry about Caspr2 antibody

 

All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

©1998-2010 Abcam plc. All rights reserved.