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All lanes : Anti-Caspr2 antibody (ab50206) at 1/1000 dilution
Lane 1 : Normal rat lung homogenate
Lane 2 : Normal rat lung plasma membrane fraction
Lane 3 : Tumor (13762) rat lung homogenate
Lane 4 : Tumor (13762) rat lung plasma membrane fraction
Lane 5 : Rat brain homogenate
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated donkey anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 148 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 seconds
The membrane was blocked with 5% milk
This image is courtesy of an anonymous Abreview
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