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Western blot - Caspr2 antibody (ab50206)
(enlarge)
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Product Name
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Caspr2 antibody
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See all Caspr2 antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Caspr2
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Immunogen
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Synthetic peptide: CDPNFTETIDESKKEWLI, corresponding to amino acids 1315-1331 of Human Caspr2, with an additional N terminal cysteine
BLAST 'CDPNFTETIDESKKEWLI' with  or 
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Reacts with
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Hu, Rat
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Predicted to react
(species key)
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Ms
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/1000. Predicted molecular weight: 148 kDa.
Note: Antibody dilutions should be made in buffer containing 1-3% bovine serum albumin.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat brain membranes
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Cellular localization
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Cell Membrane
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Research areas
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Neuroscience >> Cell Type Marker >> Glia marker >> Oligodendrocyte marker
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Relevance
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Caspr2 (for Contactin-associated protein 2)is a mammalian homolog of Drosophila Neurexin IV and belongs to the Neurexin family of highly homologous neuronal cell surface proteins; other members include neurexin I (NRXN1), neurexin II (NRXN2) and neurexin III (NRXN3) and Neurexin IV (Caspr). Caspr2 and the closely related Caspr, demarcate distinct subdomains in myelinated axons. Specifically, Caspr is present at the paranodal junctions, while Caspr2 is colocalized with Shaker-like K+ channels in the juxtaparanodal region.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.025% Sodium Azide
Constituents: 5% Sucrose, 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.300 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caspr2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Caspr2 antibody images, references, products related to ab50206 and other tools.
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Caspr2 antibody images:
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 Western blot - Caspr2 antibody (ab50206)
All lanes : Caspr2 antibody (ab50206) at 1/1000 dilution
Lane 1 : Normal rat lung homogenate
Lane 2 : Normal rat lung plasma membrane fraction
Lane 3 : Tumor (13762) rat lung homogenate
Lane 4 : Tumor (13762) rat lung plasma membrane fraction
Lane 5 : Rat brain homogenate
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated donkey anti-rabbit
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 148 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 seconds
The membrane was blocked with 5% milk This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to Caspr2
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Caspr2 antibody - more information
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Customer reviews (feedback) regarding Caspr2 antibody |
Customer FAQs regarding Caspr2 antibody |
Protocols for Caspr2 antibody |
Price and availability of products related to Caspr2 antibody |
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Caspr2 antibody - related products:
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Caspr2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Caspr2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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