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Read our guarantee »Overview
Product name
Catalase Human ELISA Kit
Specificity
Species– human, rat, mouse reactive. Others untested.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 8 | 4% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 3 | 7.3% |
Tests
1 x 96 test
Sample type
Tissue Extracts, Cell Lysate
Assay type
Sandwich
Sensitivity
4 ng/ml
Recovery
| Sample type | Average % | Range % |
|---|---|---|
| Serum | 102 | 101 - 104 |
Cross reactivity
Reacts with
Mouse, Rat, Human
Product overview
ab123456 Catalase Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Catalase in cell and tissue lysates. The assay employs an antibody specific for Human Catalase coated onto well plate strips.
Standards and samples are pipetted into the wells and analyte present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- Catalase primary detector antibody is added. After washing away unbound primary detector antibody, HRP-conjugated secondary detector antibody specific for the primary detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of analyte bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Typical working ranges:
Liver tissue extract: 0.2 - 30 µg/mL
Whole cultured cell extract: 60 - 2000 µg/mL
Human Catalase standard: 4 - 500 ng/mL
Liver tissue extract: 0.2 - 30 µg/mL
Whole cultured cell extract: 60 - 2000 µg/mL
Human Catalase standard: 4 - 500 ng/mL
Store all components at 4°C. This kit is stable for at least 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X HRP Label | 8206020 | 1 x 1ml |
| 10X Blocking Buffer | 8209802 | 1 x 6ml |
| ab110292 - 10X Catalase Detector Antibody | 8209537 | 1 x 1ml |
| 20X Buffer | 8203013 | 1 x 20ml |
| Catalase Microplate | 8209538 | 1 unit |
| Extraction Buffer | 8201093 | 1 x 15ml |
| ab91026 - Human Catalase Standard | 8209539 | 1 x 500ng |
| TMB Development Solution | 8209803 | 1 x 12ml |
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similarities
Belongs to the catalase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP04040
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAT
- CATA_HUMAN
- Catalase
Database links
- Entrez Gene: 847 Human
- SwissProt: P04040 Human
- Unigene: 502302 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use at an assay dependent concentration.
Catalase Human ELISA Kit images:
Sandwich ELISA - Catalase Human ELISA Kit (ab123456)
Example standard curve. Serially diluted recombinant Catalase in the working range of the assay.
Sandwich ELISA - Catalase Human ELISA Kit (ab123456)
Serially titrated HeLa extract in the assay working range.
Immunocytochemistry/ Immunofluorescence - Catalase Human ELISA Kit (ab123456)
Immunocytochemistry image of ab110292 stained Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the detector antibody (12C2DB9, 5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates in the peroxisomes.
Protocols
References for Catalase Human ELISA Kit (ab123456)
ab123456 has not yet been referenced specifically in any publications.
Publishing research using ab123456? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"