Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Primary antibodies
- Proteins and Peptides
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Catalase Specific Activity Assay Kit
See all Catalase products (2) ...
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 7.1% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 4 | 9.9% |
Tests
1 x 96 well plate
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Enzyme activity
Range
1 µg/ml - 1000 µg/ml
Cross reactivity
Reacts with
Mouse, Rat, Human
Product overview
ab118184, is used to determine the relative specific activity (activity and quantity) of catalase in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The assay buffer contains hydrogen peroxide which reacts with a substrate to make a luminescent product. Catalase functions rapidly to remove hydrogen peroxide from the solution and reduce the production of light. Therefore the light produced in each well is inversely proportional to the amount of catalase activity.
After activity measurement the quantity of catalase is measured by adding to each well an anti-catalase detector antibody. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of catalase bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at 4°C for upto 6 months. Refer to the protocol for further storage instructions.
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 1 x 10ml | |
| 10X Detector Antibody | 1 x 1.5ml | |
| 10X HRP Label | 1 x 1.5ml | |
| 200X Luiminescent reagent | 1 x 0.2ml | |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| 55X Coupler | 1 x 0.5ml | |
| 60X Hydrogen Peroxide | 1 x 0.5ml | |
| Base Buffer | 1 x 24ml | |
| Extraction Buffer | P/N 8201093 | 1 x 15ml |
| Microplate strips 8x12 strips | 1 unit | |
| TMB Development Solution | P/N 8209803 | 1 x 12ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Cell Damage Kits >> Oxidative stress kits
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similarities
Belongs to the catalase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP04040
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CAT
- CATA_HUMAN
- Catalase
see all
Database links
- Entrez Gene: 847 Human
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- SwissProt: P04762 Rat
- Unigene: 502302 Human
- Unigene: 4215 Mouse
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab118184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
Catalase Specific Activity Assay Kit images:
Functional Studies - Catalase Activity Assay Kit (ab118184)
Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
Functional Studies - Catalase Activity Assay Kit (ab118184)
Example standard curve for the purified human catalase sample ab91026.
Functional Studies - Catalase Activity Assay Kit (ab118184)
Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
Functional Studies - Catalase Activity Assay Kit (ab118184)
Example standard curve for the purified human catalase sample ab91026.
Functional Studies - Catalase Activity Assay Kit (ab118184)
Example relative specific activity – comparing the activity and quantity of purified erythrocyte catalase (ab91026) and catalase from human hepg2 lysate. The HepG2 sample is determined to have a higher specific activity rate.
Protocols
References for Catalase Specific Activity Assay Kit (ab118184)
ab118184 has not yet been referenced specifically in any publications.
Publishing research using ab118184? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"