Overview

  • Product name
    Catalase Specific Activity Assay Kit
    See all Catalase kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 8 7.1%
    Inter-assay
    Sample n Mean SD CV%
    1 4 9.9%
  • Sample type
    Cell culture extracts, Tissue Extracts
  • Assay type
    Enzyme activity
  • Range
    1 µg/ml - 1000 µg/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    ab118184, is used to determine the relative specific activity (activity and quantity) of catalase in a sample. The native enzyme is immunocaptured within the wells of the microplate; this removes all other enzymes. The assay buffer contains hydrogen peroxide which reacts with a substrate to make a luminescent product. Catalase functions rapidly to remove hydrogen peroxide from the solution and reduce the production of light. Therefore the light produced in each well is inversely proportional to the amount of catalase activity.

    After activity measurement the quantity of catalase is measured by adding to each well an anti-catalase detector antibody. After washing away unbound detector antibody, HRP-conjugated label specific for the detector antibody is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of catalase bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

  • Tested applications
    Suitable for: Functional Studiesmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Blocking Buffer 1 x 10ml
    10X Detector Antibody 1 x 1.5ml
    10X HRP Label 1 x 1.5ml
    200X Luiminescent reagent 1 x 0.2ml
    20X Buffer 1 x 20ml
    55X Coupler 1 x 0.5ml
    60X Hydrogen Peroxide 1 x 0.5ml
    96-Well Microplate 1 unit
    Base Buffer 1 x 24ml
    Extraction Buffer 1 x 15ml
    HRP Development Solution 1 x 12ml
  • Research areas
  • Function
    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease
    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities
    Belongs to the catalase family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localization
    Peroxisome.
  • Information by UniProt
  • Alternative names
    • CAT
    • CATA_HUMAN
    • Catalase
    • EC 1.11.1.6
    • MGC138422
    • MGC138424
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab118184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example sample curves demonstrating the working range of the assay for a human and cultured cell line lysate (HepG2).
  • Example standard curve for the purified human catalase sample ab91026.
  • Example relative specific activity – comparing the activity and quantity of purified erythrocyte catalase (ab91026) and catalase from human hepg2 lysate. The HepG2 sample is determined to have a higher specific activity rate.

Protocols

References

This product has been referenced in:
  • Padovani D  et al. Sulfheme formation during homocysteine S-oxygenation by catalase in cancers and neurodegenerative diseases. Nat Commun 7:13386 (2016). Functional Studies ; Human . Read more (PubMed: 27848965) »

See 1 Publication for this product

Customer reviews and Q&As

Vielen Dank für Ihre Anfrage.

Laut unserem Labor ist es völlig in Ordnung, wenn Sie gefrorenes Material verwenden, da wir dies teilweise auch in unserem Labor verwenden.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up