Anti-Catalase antibody [12C2DB9 ] - Peroxisome Marker (ab110292)

Overview

• Product nameAnti-Catalase antibody [12C2DB9 ] - Peroxisome MarkerSee all Catalase primary antibodies ...
• Description
  Mouse monoclonal [12C2DB9 ] to Catalase - Peroxisome Marker
• Tested applicationsICC, IP, Flow Cyt, In-Cell ELISA more details
• Species reactivity
  Reacts with: Rat, Cow, Human
  
• Immunogen

  Rat liver mitochondria

• Positive controlHeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver

Properties

• FormLiquid
• Storage instructionsStore at +4°C. Do not freeze.
• Storage bufferPreservative: 0.02% Sodium azide
  Constituent: HBS
• Concentration information loading...
• PurityAmmonium Sulphate Precipitation
• Purification notesPurity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
• Clonality Monoclonal
• Clone number12C2DB9
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Antioxidants

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

  • Cardiovascular
  • Heart
  • Cardiac metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Peroxisome

Applications

Target

• FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
• Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
• Sequence similaritiesBelongs to the catalase family.
• Post-translational
  modificationsThe N-terminus is blocked.
• Cellular localizationPeroxisome.

Target information above from: UniProt accession P04040 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 531682 Cow
  • Entrez Gene: 847 Human
  • Entrez Gene: 24248 Rat
  • Omim: 115500 Human
  • SwissProt: P00432 Cow
  • SwissProt: P04040 Human
  • SwissProt: P04762 Rat
  • Unigene: 502302 Human

  • Unigene: 3001 Rat

  see all

• Alternative names
  Cas1 antibodyCAT antibodyCATA_HUMAN antibody

  Catalase antibodyCs1 antibodyMGC138422 antibodyMGC138424 antibody

  see all

Anti-Catalase antibody [12C2DB9 ] - Peroxisome Marker images

• 

  Immunocytochemistry - Anti-Catalase antibody [12C2DB9 ] (ab110292)
  Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
• 

  Immunoprecipitation - Anti-Catalase antibody [12C2DB9 ] (ab110292)
  Immunoprecipitation of Catalase using ab110292 from:
  Lane 1: HepG2 cells
  Lane 2: Cow heart sample
  Lane 3: Rat liver sample
  
  Predicted band size is 60 kDa.
• 

  Flow Cytometry - Catalase antibody [12C2DB9 ] (ab110292)
  Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).