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Recombinant fragment. within Mouse Catalase aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P24270
ab223793 is a PBS-only buffer version of ab209211.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab223793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).|
|Flow Cyt||Use at an assay dependent concentration.
ab199376-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
This WB data was generated using the same anti-Catalase antibody clone [EPR20198] in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (cat# ab209211).
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CAT knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hek293 whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab209211 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab209211 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab223793 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"