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Product name
Anti-Catalase antibody - Peroxisome Marker
See all Catalase products (22) ...
Description
Rabbit polyclonal to Catalase - Peroxisome Marker
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Chicken, Human
Immunogen
Catalase (Bovine Liver)
Positive control
This antibody gave a positive control in the following Tissue Lysates: Human Liver, Mouse Liver, Rat Liver, Chicken Liver
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Redox metabolism >> Antioxidants
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cardiovascular >> Heart >> Cardiac metabolism
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab15834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml.
WB: Use a concentration of 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 60 kDa).
Target
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similarities
Belongs to the catalase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP04040
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Database links
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- SwissProt: P04762 Rat
- Unigene: 502302 Human
see all
Anti-Catalase antibody - Peroxisome Marker images:
Western blot - Catalase antibody (ab15834)
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 : Catalase at 0.1 µg
Lane 2 : Human Liver lysate at 20 µg
Lane 3 : Mouse Liver lysate at 20 µg
Lane 4 : Rat Liver lysate at 20 µg
Lane 5 : Chicken Liver lysate at 20 µg
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 60 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Western blot
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :
Lane 2 :
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
Immunocytochemistry/ Immunofluorescence - Catalase antibody - Peroxisome Marker (ab15834)
ICC/IF image of ab15834 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab15834, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
References for Anti-Catalase antibody - Peroxisome Marker (ab15834)
This product has been referenced in:
- Tang D et al. Endogenous HMGB1 regulates autophagy. J Cell Biol 190:881-92 (2010). WB; Human.Read more (PubMed: 20819940) »
- Habib LK et al. Inhibitors of catalase-amyloid interactions protect cells from beta-amyloid-induced oxidative stress and toxicity. J Biol Chem 285:38933-43 (2010). ICC/IF; Human.Read more (PubMed: 20923778) »
See all 4 publications for this product
Publishing research using ab15834? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"