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Product name
Anti-Catalase antibody - Peroxisome Marker
See all Catalase products (3) ...
Description
Rabbit polyclonal to Catalase - Peroxisome Marker
Tested applications
IHC-Fr, IHC-P, ICC, WB, IP, ELISA, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Chicken, Cow, Human
Immunogen
Catalase [Bovine Liver]
Positive control
ICC: MDCK cells, COS-1 cells (see review) IHC-P: rat or mouse liver sections (see review) WB: rat thoracic aorta and vena cava lysate (see review)
General notes
This product has been assayed against 1.0 ug of Catalase [Bovine liver] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:30,000 to 1:150,000 is suggested for this product.Storage: Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.
Properties
Form
Liquid
Storage instructions
Please see Notes section
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Preadsorbed IgG fraction
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Catalase [Bovine liver].
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cardiovascular >> Heart >> Cardiac metabolism
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: 1/200 - 1/500. See Abreviews.
ICC: 1/300.
WB: 1/1000 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IP: Use at an assay dependent concentration.
ELISA: 1/60000 - 1/260000.
ICC/IF: 1/300. See Abreviews. Fix in 4%PFA/20mM PIPES pH6.8/100mM NaCl/30mM Glucose/2mM MgCl2/1mM EGTA.
Target
Relevance
Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes.
Alternative names
- Cas 1 antibody
- CAT antibody
- Cs1 antibody
see all
Database links
- Entrez Gene: 423600 Chicken
- Entrez Gene: 280743 Cow
- Entrez Gene: 847 Human
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P00432 Cow
- SwissProt: P04040 Human
- SwissProt: Q2M1U4 Human
see all
Anti-Catalase antibody - Peroxisome Marker images:
Immunocytochemistry/ Immunofluorescence - Catalase antibody - Peroxisome Marker (ab1877)
ab1877 at a dilution of 1/200 staining Hydrogen peroxide treated HeLa cells (1mM, 1hr) by immunocytochemistry. The antibody was incubated with the cells for 2 hours and then detected using a Cy5 conjugated anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Jinhee Kim on 30 January 2006.
Western blot - Catalase antibody - Peroxisome Marker (ab1877)
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab1877) at 1/5000 dilution
Lane 1 : 3ug liver 1 (Homogenate)
Lane 2 : 3ug liver 1 (PNS)
Lane 3 : 3ug liver 1 (Float 1)
Lane 4 : 3ug liver 1 (Float 2)
Lane 5 : 3ug liver 1(Interface - enriched fraction)
Lane 6 : 3ug liver 2 (Homogenate)
Lane 7 : 3ug liver 2 (PNS)
Lane 8 : 3ug liver 2 (Float 1)
Lane 9 : 3ug liver 2 (Float 2)
Lane 10 : 3ug liver 2(Interface - enriched fraction)
Secondary
HRP conjugated goat polyclonal antibody
developed using the ECL technique
Predicted band size : 59 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Additional bands at : <25 kDa (possible degradation product).
Exposure time : 5 seconds
This image is courtesy of an anonymous Abreview
Western blot - Catalase antibody - Peroxisome Marker (ab1877)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
ab1877 at 1/500 staining human placenta (whole tissue lysate) by WB (20ug/lane). An HRP donkey anti-rabbit antibody was used as the secondary. The image shows 6 samples each from normal and preeclamptic placenta.
This image is courtesy of an Abreview submitted by Dr Rose Webster
Immunoprecipitation - Anti-Catalase antibody - Peroxisome Marker (ab1877)
Catalase was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Catalase (ab1877) and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab1877. Secodary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Bands: 60kDa: Catalase
Protocols
References for Anti-Catalase antibody - Peroxisome Marker (ab1877)
This product has been referenced in:
- Berg RK et al. Genomic HIV RNA induces innate immune responses through RIG-I-dependent sensing of secondary-structured RNA. PLoS One 7:e29291 (2012). ICC/IF; Human.Read more (PubMed: 22235281) »
- Panov AV et al. Metabolic and functional differences between brain and spinal cord mitochondria underlie different predisposition to pathology. Am J Physiol Regul Integr Comp Physiol 300:R844-54 (2011).Read more (PubMed: 21248309) »
See all 38 publications for this product
Publishing research using ab1877? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"