Anti-Catalase antibody (ab88067)

Overview

• Product nameAnti-Catalase antibodySee all Catalase primary antibodies ...
• Description
  Mouse monoclonal to Catalase
• Tested applicationsWB, ELISA, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 1-101 of Human Catalase with a 26 kDa tag

• Positive controlHepG2 cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Antioxidants

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Heat Shock Proteins

  • Cardiovascular
  • Heart
  • Cardiac metabolism

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Peroxisome

Applications

Target

• FunctionOccurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
• Involvement in diseaseDefects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
• Sequence similaritiesBelongs to the catalase family.
• Post-translational
  modificationsThe N-terminus is blocked.
• Cellular localizationPeroxisome.

Target information above from: UniProt accession P04040 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 847 Human
  • Omim: 115500 Human
  • SwissProt: P04040 Human
  • Unigene: 502302 Human

• Alternative names
  Cas1 antibodyCAT antibodyCATA_HUMAN antibody

  Catalase antibodyCs1 antibodyMGC138422 antibodyMGC138424 antibody

  see all

Anti-Catalase antibody images

• 

  Western blot - Catalase antibody [2G6] (ab88067)
  Anti-Catalase antibody (ab88067) at 1 µg/ml + immunogen (recombinant fragment) at 0.2 µg
  
  Secondary
  Goat anti-mouse IgG at 1/5000 dilution
  
  Predicted band size : 60 kDa
  
• 

  Western blot - Catalase antibody [2G6] (ab88067)
  Anti-Catalase antibody (ab88067) at 1 µg/ml + HepG2 cell lysate at 50 µg
  
  Secondary
  Goat anti mouse IgG at 1/5000 dilution
  
  Predicted band size : 60 kDa
  Observed band size : 60 kDa
  
• 

  Flow Cytometry-Anti-Catalase antibody(ab88067)
  Overlay histogram showing HeLa cells stained with ab88067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab88067, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.