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Product name
Catalase protein (Active)
See all Catalase products (3) ...
Protein description
Full length native Human Catalase purified from Human Erythrocytes; amino acids 2-527, Homotetramer 244kDa (subunit 59.7kDa). UniProt P04040.
Expression host
Native
Properties
Purity
> 95% by SDS-PAGE
Biological activity
Activity: Greater than 50,000 units per mg protein. One unit is equal to the amount of enzyme that will decompose one micromole of hydrogen peroxide per minute at 25°C, pH 7.0
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50mM Tris HCl, pH 8.0
Concentration
Concentration information loading...
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 1.39.
Prepared from whole blood shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Redox metabolism >> Antioxidants
Cancer >> Cancer Metabolism >> Cellular metabolic process
Cardiovascular >> Heart >> Cardiac metabolism
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Peroxisome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab91026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. Ab91026 can be used as a WB positive control in conjunction with ab15834.
FuncS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
Sequence similarities
Belongs to the catalase family.
Post-translational
modifications
The N-terminus is blocked.
Cellular localization
Peroxisome.
Target information above from: UniProt accessionP04040
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Catalase protein (Active) images:
SDS-PAGE - Catalase protein (Active) (ab91026)
SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1: 5 µg Catalase (reduced/heated)
Lane 2: 10 µg Catalase (reduced/heated)
Lane 3: 20 µg Catalase (reduced/heated)
Lane 4: Molecular weight markers
Lane 5: 5 µg Catalase (non-reduced/no heat)
Lane 6: 10 µg Catalase (non-reduced/no heat)
Lane 7: 20 µg Catalase (non-reduced/no heat)
Western blot
All lanes : Anti-Catalase antibody - Peroxisome Marker (ab15834) at 5 µg/ml
Lane 1 :
Lane 2 :
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 10 seconds
References for Catalase protein (Active) (ab91026)
ab91026 has not yet been referenced specifically in any publications.
Publishing research using ab91026? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"