Anti-Cathelicidin antibody (ab72012)

Overview

• Product nameAnti-Cathelicidin antibodySee all Cathelicidin primary antibodies ...
• Description
  Sheep polyclonal to Cathelicidin
• SpecificityThis antibody appears to be specific for Cathelicidin.
• Tested applicationsWB, ICC/IF, ELISA, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A synthetic peptide from part of human Cathelicidin, conjugated to an immunogenic carrier protein.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Lipopolysaccharide
  • Bacterial Lipopolysaccharide

  • Immunology
  • Secreted Molecules
  • Other secreted molecules

Applications

Target

• FunctionBinds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
• Tissue specificityExpressed in bone marrow and testis and neutrophils.
• Sequence similaritiesBelongs to the cathelicidin family.
• Post-translational
  modificationsThe N-terminus is blocked.
• Cellular localizationSecreted.

Target information above from: UniProt accession P49913 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 820 Human
  • Omim: 600474 Human
  • SwissProt: P49913 Human
  • Unigene: 51120 Human

• Alternative names
  18 kDa cationic antimicrobial protein antibodyAntibacterial protein FALL-39 antibodyAntibacterial protein LL-37 antibody

  CAMP antibodyCAMP_HUMAN antibodyCAP 18 antibodyCAP-18 antibodyCAP18 antibodyCathelicidin antimicrobial peptide antibodyCathelicidin antimicrobial peptide precursor antibodyCathelin-like protein antibodyCathelin-related antimicrobial peptide antibodyCATHL3 antibodyCationic antimicrobial protein CAP 18 antibodyCationic antimicrobial protein, 18-KD antibodyCLP antibodyCnlp antibodyCramp antibodyCRAMP, mouse, homolog of antibodyFALL 39 antibodyFALL 39 peptide antibiotic antibodyFALL-39 peptide antibiotic antibodyFALL39 antibodyhCAP 18 antibodyhCAP-18 antibodyhCAP18 antibodyHSD26 antibodyLL 37 antibodyLL37 antibodyMCLP antibodymcramp antibodyPeptide antibiotic, PR-39, porcine, homolog of antibodySOB3 antibody

  see all

Anti-Cathelicidin antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Cathelicidin antibody (ab72012)
  Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with ab72012, diluted 50µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
• 

  Western blot - Cathelicidin antibody (ab72012)
  Lane 1 : Anti-Cathelicidin antibody (ab72012) at 50 µg/ml
  Lane 2 : Anti-Cathelicidin antibody (ab72012) at 50 µg/ml (pre-absorbed with immunising peptide)
  
  Lane 1 : Whole cell lysate prepared from human neutrophils
  Lane 2 : Whole cell lysate prepared from human neutrophils
  
  
  Predicted band size : 19 kDa