Anti-Cathelicidin antibody (ab72012)

Overview

  • Product nameAnti-Cathelicidin antibodySee all Cathelicidin primary antibodies ...
  • Description
    Sheep polyclonal to Cathelicidin
  • SpecificityThis antibody appears to be specific for Cathelicidin.
  • Tested applicationsWB, ICC/IF, ELISA, Flow Cyt more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide from part of human Cathelicidin, conjugated to an immunogenic carrier protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab72012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 10 - 50 µg/ml. Predicted molecular weight: 19 kDa.
ICC/IF Use a concentration of 50 µg/ml.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionBinds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
  • Tissue specificityExpressed in bone marrow and testis and neutrophils.
  • Sequence similaritiesBelongs to the cathelicidin family.
  • Post-translational
    modifications
    The N-terminus is blocked.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P49913 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 18 kDa cationic antimicrobial protein antibody
    • Antibacterial protein FALL-39 antibody
    • Antibacterial protein LL-37 antibody
    • CAMP antibody
    • CAMP_HUMAN antibody
    • CAP 18 antibody
    • CAP-18 antibody
    • CAP18 antibody
    • Cathelicidin antimicrobial peptide antibody
    • Cathelicidin antimicrobial peptide precursor antibody
    • Cathelin-like protein antibody
    • Cathelin-related antimicrobial peptide antibody
    • CATHL3 antibody
    • Cationic antimicrobial protein CAP 18 antibody
    • Cationic antimicrobial protein, 18-KD antibody
    • CLP antibody
    • Cnlp antibody
    • Cramp antibody
    • CRAMP, mouse, homolog of antibody
    • FALL 39 antibody
    • FALL 39 peptide antibiotic antibody
    • FALL-39 peptide antibiotic antibody
    • FALL39 antibody
    • hCAP 18 antibody
    • hCAP-18 antibody
    • hCAP18 antibody
    • HSD26 antibody
    • LL 37 antibody
    • LL37 antibody
    • MCLP antibody
    • mcramp antibody
    • Peptide antibiotic, PR-39, porcine, homolog of antibody
    • SOB3 antibody
    see all

Anti-Cathelicidin antibody images

  • Staining of a cytospin preparation of peripheral blood mononuclear cells isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with ab72012, diluted 50µg/ml: in blocking buffer, for 1 hour at room temperature. Cells were washed twice in PBS followed by incubation for 30 minutes with the FITC-labeled secondary antibody. Finally, cells were washed and nuclear counterstained with DAPI. The antibody selectively recognizes polymorphonuclear cells.
  • Lane 1 : Anti-Cathelicidin antibody (ab72012) at 50 µg/ml
    Lane 2 : Anti-Cathelicidin antibody (ab72012) at 50 µg/ml (pre-absorbed with immunising peptide)

    Lane 1 : Whole cell lysate prepared from human neutrophils
    Lane 2 : Whole cell lysate prepared from human neutrophils


    Predicted band size : 19 kDa

References for Anti-Cathelicidin antibody (ab72012)

ab72012 has not yet been referenced specifically in any publications.

Product Wall

ab72012 is raised against LL-37 which is the active part of the whole molecule (CAP-18) from aa134-170.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"