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Western blot - Cathelicidin antibody [mAbcam 58387] (ab58387)
![Western blot - Cathelicidin antibody [mAbcam 58387] (ab58387)](/ps/datasheet/images/58/ab58387/Cathelicidin-Primary-antibodies-ab58387-1.jpg)
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Product Name
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Cathelicidin antibody [mAbcam 58387]
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See all Cathelicidin antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [mAbcam 58387] to Cathelicidin
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Cathelicidin.
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Glla, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 10 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in human spleen tissue lysate.
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Cellular localization
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Secreted
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Research areas
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Immunology >> Secreted Molecules >> Other secreted molecules
Microbiology >> Lipopolysaccharide >> Bacterial Lipopolysaccharide
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Relevance
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Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase negative granules of neutrophils. The cathelicidins are synthesized as preproproteins. Within neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide. The active biologic domains of the cathelicidins generally reside in the C terminus. The C terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules.
CAP18 is the only human cathelicidin found so far and closely resembles CRAMP, the sole member of this gene family in mice. LL-37 is the 37 amino acid C-terminal antibacterial protein.
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Cathelicidin antibody [mAbcam 58387] (ab58387) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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mAbcam 58387
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cathelicidin antibody [mAbcam 58387] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cathelicidin antibody [mAbcam 58387] images, references, products related to ab58387 and other tools.
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Cathelicidin antibody [mAbcam 58387] images:
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 Western blot - Cathelicidin antibody [mAbcam 58387] (ab58387)
Cathelicidin antibody [mAbcam 58387] (ab58387) at 10 µg/ml + Spleen (Human) Tissue Lysate - adult normal tissue (ab29699) at 20 µg
Secondary
Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 19 kDa
Observed band size : 19 kDa
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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Search PubMed (MEDLINE) for references to Cathelicidin
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Cathelicidin antibody [mAbcam 58387] - more information
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Customer reviews (feedback) regarding Cathelicidin antibody [mAbcam 58387] |
Customer FAQs regarding Cathelicidin antibody [mAbcam 58387] |
Protocols for Cathelicidin antibody [mAbcam 58387] |
Price and availability of products related to Cathelicidin antibody [mAbcam 58387] |
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Cathelicidin antibody [mAbcam 58387] - related products:
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Cathelicidin antibody [mAbcam 58387] - other tools:
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Contact Abcam with a Technical Enquiry about Cathelicidin antibody [mAbcam 58387] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Cathelicidin antibody [mAbcam 58387] (ab58387)](/index.html?datasheet=58387#Cathelicidin-Primary-antibodies-ab58387-1.jpg)
