Overview
- Product nameCathepsin B Human ELISA KitSee all Cathepsin B kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 5 pg/ml
- Range156 pg/ml - 10000 pg/ml
- Species reactivityReacts with: Human
- Product overview
ab119584 Cathepsin B Human ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Cathepsin B specific-specific monoclonal antibodies were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin B amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-human Cathepsin B antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-human Cathepsin B antibody 1 x 130µl Lyophilized recombinant human Cathepsin B standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml - Research Areas
Target information above from: UniProt accession
P07858
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- aAmyloid precursor protein secretaseAPP secretaseAPPS
- CATB_HUMANCathepsin B heavy chainCathepsin B1CPSBCTSBCysteine protease
see all
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
Applications
Our Abpromise guarantee covers the use of ab119584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Cathepsin B Human ELISA Kit images
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Sandwich ELISA - Cathepsin B Human ELISA Kit (ab119584)Representative standard curve using ab119584.
Protocols
References for Cathepsin B Human ELISA Kit (ab119584)
ab119584 has not yet been referenced specifically in any publications.
