Overview

  • Product nameCathepsin B Human ELISA KitSee all Cathepsin B kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Cathepsin B in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin B in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin B specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin B is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin B amount of sample captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Sequence similaritiesBelongs to the peptidase C1 family.
  • Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07858 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • aAmyloid precursor protein secretase
    • APP secretase
    • APPS
    • CATB_HUMAN
    • Cathepsin B heavy chain
    • Cathepsin B1
    • CPSB
    • CTSB
    • Cysteine protease
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Cathepsin B Human ELISA Kit images

  • Representative Standard Curve Using ab119584.

Protocols

References for Cathepsin B Human ELISA Kit (ab119584)

This product has been referenced in:
  • Zhu S  et al. Inhibiting autophagy potentiates the anticancer activity of IFN1@/IFNa in chronic myeloid leukemia cells. Autophagy 9:317-27 (2013). Read more (PubMed: 23242206) »

See 1 Publication for this product

Product Wall



J’ai eu une réponse du laboratoire qui m’a confirmé que les plaques sont divisibles. La plaque peut être divisé en 12 donc vous pouvez tester 8 échantillons à chaque fois.

Une foi...

Read More

Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

I am very sorry to confirm that there are no discounts available that I can provide on this occasion. I can let you know that if...

Read More

Thank you for contacting us and sorry for the delay in getting back to you.

I can confirm that the Cathepsin B Human ELISA Kit (ab119584) can detect both the mature form and uncleaved form of Cathepasin B.

The kit has been found to ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"