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Products:Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins
Overview
Product name
Anti-Cathepsin B antibody - Catalytic domain
See all Cathepsin B products (11) ...
Description
Rabbit polyclonal to Cathepsin B - Catalytic domain
Specificity
ab49232 recognises cathepsin B in the catalytic domain region of the heavy chain.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the heavy chain in the catalytic domain of human cathepsin B.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins
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Western blot - Cathepsin B antibody - Catalytic domain (ab49232)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 38 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence similarities
Belongs to the peptidase C1 family.
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accessionP07858
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Database links
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
Anti-Cathepsin B antibody - Catalytic domain images:
Western blot - Cathepsin B antibody - Catalytic domain (ab49232)
All lanes : Anti-Cathepsin B antibody - Catalytic domain (ab49232) at 1/1000 dilution
Lane 1 : Recombinant Human Cathepsin B at 0.04 µg
Lane 2 : Recombinant Human Cathepsin B at 0.02 µg
Lane 3 : Recombinant Human Cathepsin B at 0.01 µg
Lane 4 : Recombinant Human Cathepsin B at 0.004 µg
Lane 5 : Recombinant Human Cathepsin B at 0.002 µg
Predicted band size : 38 kDa
Observed band size : 34 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-Cathepsin B antibody - Catalytic domain (ab49232)
ab49232 has not yet been referenced specifically in any publications.
Publishing research using ab49232? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"