Anti-Cathepsin B antibody (ab92955)

Overview

• Product nameAnti-Cathepsin B antibodySee all Cathepsin B primary antibodies ...
• Description
  Rabbit polyclonal to Cathepsin B
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Orangutan
• Immunogen

  Synthetic peptide derived from within residues 200 - 300 of Human Cathepsin B.

  Read Abcam's proprietary immunogen policy

  Synthetic peptide of Human Cathepsin B.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in the following lysates: Human Liver Tissue; Human Kidney Tissue; Human Brain Tissue; Human Placenta Tissue; HepG2 Whole Cell; HEK293 Whole Cell Lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • ECM
  • Cell adhesion
  • Other

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • Other Enzymes

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

Applications

Target

• FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
• Sequence similaritiesBelongs to the peptidase C1 family.
• Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accession P07858 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1508 Human
  • Omim: 116810 Human
  • SwissProt: P07858 Human
  • Unigene: 520898 Human

• Alternative names
  Amyloid precursor protein secretase antibodyAPP secretase antibodyAPPS antibody

  CATB_HUMAN antibodyCathepsin B heavy chain antibodyCathepsin B1 antibodyCathepsinB antibodyCPSB antibodyCTSB antibodycysteine protease antibodyOTTHUMP00000116009 antibodyOTTHUMP00000229510 antibodyOTTHUMP00000229511 antibodyOTTHUMP00000229512 antibodyOTTHUMP00000229514 antibodyOTTHUMP00000229515 antibodyOTTHUMP00000229516 antibodyPreprocathepsin B antibody

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Anti-Cathepsin B antibody images

• 

  Western blot - Cathepsin B antibody (ab92955)
  All lanes : Anti-Cathepsin B antibody (ab92955) at 1 µg/ml
  
  Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
  Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
  Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
  Lane 4 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
  Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 25,30 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 30 seconds
• 

  Immunocytochemistry/ Immunofluorescence - Cathepsin B antibody (ab92955)

  ICC/IF image of ab92955 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab92955 at 1µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.