Cathepsin D Activity Assay Kit (Fluorometric) (ab65302)
- Product nameCathepsin D Activity Assay Kit (Fluorometric)See all Cathepsin D kits ...
- Tests100 x 1 assay
- Sample typeTissue Extracts, Cell Lysate
- Assay typeQuantitative
- Assay time2h 00m
- Product overview
Abcam's Cathepsin D Activity Assay Kit is a fluorescence-based assay that utilizes the preferred cathepsin-D substrate sequence GKPILFFRLK(Dnp)-D-R-NH2) labeled with MCA. Cell lysates or other samples that contain cathepsin-D will cleave the synthetic substrate to release fluorescence, which can then easily be quantified using a fluorometer or fluorescence plate reader at Ex/Em = 328/460 nm.
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Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests CD Cell Lysis Buffer WM 1 x 25ml CD Reaction Buffer NM 1 x 5ml CD Substrate (1mM) Brown 1 x 200µl
- Research Areas
- CLN10CPSDCTSDLysosomal aspartyl protease
Our Abpromise guarantee covers the use of ab65302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Cathepsin D Activity Assay Kit (Fluorometric) images
Cathepsin D assays were performed using various numbers of Jurkat Cells as indicated. Results were analyzed by fluorescence plate reader according to the kit instructions.
References for Cathepsin D Activity Assay Kit (Fluorometric) (ab65302)
This product has been referenced in:
- Moles A et al. Cathepsin B overexpression due to Acid sphingomyelinase ablation promotes liver fibrosis in niemann-pick disease. J Biol Chem 287:1178-88 (2012). Read more (PubMed: 22102288) »
- Dehay B et al. Loss of P-type ATPase ATP13A2/PARK9 function induces general lysosomal deficiency and leads to Parkinson disease neurodegeneration. Proc Natl Acad Sci U S A 109:9611-6 (2012). Read more (PubMed: 22647602) »