Overview

  • Product nameCathepsin D Human ELISA Kit
  • Detection methodColorimetric
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    156 pg/ml - 10000 pg/ml
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human Cathepsin D in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin D in cell culture supernatants, serum and plasma (heparin, EDTA).

    A Cathepsin D specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin D is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin D amount of sample captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • Involvement in diseaseDefects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.
  • Sequence similaritiesBelongs to the peptidase A1 family.
  • Cellular localizationLysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • CATD_HUMAN
    • Cathepsin D heavy chain
    • Ceroid lipofuscinosis neuronal 10
    • CLN10
    • CPSD
    • ctsd
    • Lysosomal aspartyl protease
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Cathepsin D Human ELISA Kit images

  • Representative Standard Curve using ab119586.

Protocols

References for Cathepsin D Human ELISA Kit (ab119586)

ab119586 has not yet been referenced specifically in any publications.

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Either washing or blotting is effective. In this assay, blotting works the best and it is validated. Please follow the protocol.
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