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Anti-Cathepsin D antibody [4G2] (ab40697)

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Overview

Product name

Anti-Cathepsin D antibody [4G2]
See all Cathepsin D products (15) ...

Description

Mouse monoclonal [4G2] to Cathepsin D

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment, corresponding to amino acids 21-412 of Human Cathepsin D

Positive control

HepG2, Hep3B, MDA-MB-231 and MCF7 cell lysates

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

4G2

Myeloma

Sp2/0

Isotype

IgG2b

Light chain type

kappa

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Cathepsins
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Neuroscience >> Cell Adhesion Proteins >> Membrane Proteins

  • Western blot - Cathepsin D antibody [4G2] (ab40697)Western blot - Cathepsin D antibody [4G2] (ab40697) image (enlarge)

  • Western blot Western blot image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab40697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Involvement in disease

Defects in CTSD are the cause of neuronal ceroid lipofuscinosis type 10 (CLN10) [MIM:610127]; also known as neuronal ceroid lipofuscinosis due to cathepsin D deficiency. A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.

Sequence similarities

Belongs to the peptidase A1 family.

Cellular localization

Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Target information above from: UniProt accessionP07339 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CatD antibody
  • CATD_HUMAN antibody
  • Cathepsin D heavy chain antibody
  • CathepsinD antibody
  • CD antibody
  • CLN10 antibody
  • CPSD antibody
  • CTSD antibody
  • Lysosomal aspartyl peptidase antibody
  • MGC2311 antibody
see all

Database links

Anti-Cathepsin D antibody [4G2] images:

  Western blot - Cathepsin D antibody [4G2] (ab40697)

Western blot - Cathepsin D antibody [4G2] (ab40697)

All lanes : Anti-Cathepsin D antibody [4G2] (ab40697) at 1/1000 dilution

Lane 1 : HepG2 cell lysate
Lane 2 : Hep3B cell lysate
Lane 3 : MDA-MB-231 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

developed using the ECL technique

Observed band size : 45 kDa (why is the actual band size different from the predicted?)

  Western blot

Western blot

Anti-Cathepsin D antibody [4G2] (ab40697) at 1/500 dilution + Cathepsin D protein (Active) (ab91123) at 0.1 µg

Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 30 seconds

References for Anti-Cathepsin D antibody [4G2] (ab40697)

This product has been referenced in:

  • Broadstock M  et al. Synaptic protein expression is regulated by a pro-oxidant diet in APPxPS1 mice. J Neural Transm : (2011). WB; Mouse.Read more (PubMed: 22057511) »
  • McKeen HD  et al. FKBPL regulates estrogen receptor signaling and determines response to endocrine therapy. Cancer Res 70:1090-100 (2010). WB; Human.Read more (PubMed: 20103631) »

See all 2 publications for this product

Publishing research using ab40697? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"