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Cathepsin G Activity Assay Kit (Colorimetric) (ab126780)

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Overview

  • Product nameCathepsin G Activity Assay Kit (Colorimetric)
  • Tests
    1 x 100 test
  • Sample type
    Serum, Tissue Extracts, Cell Lysate
  • Assay typeQuantitative
  • Range
    0 nmol/well - 50 nmol/well
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: all Mammals
  • Product overview

    In Abcam's Cathepsin G Activity Assay Kit (Colorimetric), Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

  • Tested applicationsFunctional Studies more details

Properties

  • FunctionSerine protease with trypsin- and chymotrypsin-like specificity. Cleaves complement C3. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, antibacterial activity is inhibited by LPS from P.aeruginosa, Z-Gly-Leu-Phe-CH2Cl and phenylmethylsulfonyl fluoride.
  • Sequence similaritiesBelongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localizationCell surface.

    • Target information above from: UniProt accession P08311 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CATG_HUMANCathepsin GCGCtsg

    Applications

    Our Abpromise guarantee covers the use of ab126780 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Cathepsin G Activity Assay Kit (Colorimetric) images

    • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
      Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
    • Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
      Functional Studies - Cathepsin G Activity Assay Kit (ab126780)
      p-NA Standard Curve. Performed according to assay protocol.

    Protocols

    References for Cathepsin G Activity Assay Kit (Colorimetric) (ab126780)

    ab126780 has not yet been referenced specifically in any publications.

    Publishing research using ab126780 ? Please let us know so that we can cite the reference in this datasheet

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