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Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)

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Overview

  • Product nameCathepsin H 293T Transfected Lysate - (positive control)See all Cathepsin H lysates ...
  • General notesab94088 is a 293T cell transfected lysate in which Human Cathepsin H has been transiently over-expressed using a pCMV-Cathepsin H plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundThe cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer.

    • Applications

    Our Abpromise guarantee covers the use of ab94088 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Cathepsin H 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)
      SDS-PAGE - Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)
      ab94088 at 15µg/lane on an SDS-PAGE gel
    • Western blot - Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)
      Western blot - Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)
      All lanes : Anti-Cathepsin H antibody (ab88683) at 1/500 dilution

      Lane 1 : Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Cathepsin H 293T Transfected Lysate - (positive control) (ab94088)

    ab94088 has not yet been referenced specifically in any publications.

    Publishing research using ab94088 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"