Cathepsin L Human ELISA Kit (with color giving dyes) (ab119509)
Overview
- Product nameCathepsin L Human ELISA Kit (with color giving dyes)
- Precision
Intra-assay Sample n Mean SD CV% Overall 6.2% Inter-assay Sample n Mean SD CV% Overall 6% - Tests1 x 96 test
- Sample typeCell culture supernatant, Serum
- Assay typeSandwich
- Sensitivity1.7 ng/ml
- Range3.1 ng/ml - 50 ng/ml
- Recovery
92 %
Sample specific recovery Sample type Average % Range Serum 92 78% - 113% - Species reactivityReacts with: Human
- Product overview
ab119509 Cathepsin L Human ELISA Kit (with color giving dyes) is an enzyme-linked immunosorbent assay for the quantitative detection of human Cathepsin L. ab119509 is for research use only. Not for diagnostic or therapeutic procedures.
Cathepsin L is closely related to the recently described cathepsin V (L2) and the sequence indentity of almost 80% between them requires antibodies for the detection of cathepsin L which do not crossreact with cathepsin V. This requirement can only be met by epitope specific monoclonal antibodies as are used in ab119509.
Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration
In order to help avoid any mistakes in pipetting, our kit provides color giving dyes that help monitor the addition of a solution to the reaction. Each dye gives a distinctive color for each step of the ELISA procedure.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests Adhesive Films 4 units Assay Buffer Concentrate (20x) 1 x 50ml Biotin-Conjugate anti-human Cathepsin L monoclonal antibody 1 x 100µl Blue-Dye 1 x 0.4ml Green-Dye 1 x 0.4ml Human Cathepsin L Standard 2 vials Monoclonal anti-human Cathepsin L-coated microwell plate 1 unit Red-Dye 1 x 0.4ml Sample Diluent 1 x 12ml Stop Solution 1 x 15ml Streptavidin-HRP 1 x 200µl Substrate Solution 1 x 15ml Wash Buffer Concentrate (20x) 1 x 50ml - Research Areas
Target information above from: UniProt accession
P07711
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Cathepsin L1Cathepsin L1 light chainCATL
- CATL1_HUMANCTSL1Major excreted proteinMEP
see all
- Entrez Gene: 1514 Human
- Omim: 116880 Human
- SwissProt: P07711 Human
- Unigene: 418123 Human
Applications
Our Abpromise guarantee covers the use of ab119509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Cathepsin L Human ELISA Kit (with color giving dyes) images
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Sandwich ELISA - Cathepsin L Human ELISA Kit (with color giving dyes) (ab119509)Representative standard curve using ab119509.
Protocols
References for Cathepsin L Human ELISA Kit (with color giving dyes) (ab119509)
ab119509 has not yet been referenced specifically in any publications.
