Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Adaptive Immunity >> MHC >> Class II
Overview
Product name
Anti-Cathepsin S antibody - Catalytic domain
See all Cathepsin S products (9) ...
Description
Rabbit polyclonal to Cathepsin S - Catalytic domain
Specificity
We have a range of domain specific antibodies for this target. For a full list please see all Cathepsin S antibodies. This antibody reacts with Cathepsin S.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide based on the catalytic domain of human Cathepsin S.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Cathepsins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Immunology >> Cell Type Markers >> Non-CD >> APC
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Immunology >> Adaptive Immunity >> MHC >> Class II
-
Western blot - Cathepsin S antibody - Catalytic domain (ab50400)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 37 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene.
Cellular localization
Lysosomal.
Alternative names
- CathepsinS antibody
- CTSS antibody
Database links
- Entrez Gene: 1520 Human
- Omim: 116845 Human
- SwissProt: P25774 Human
- Unigene: 181301 Human
Anti-Cathepsin S antibody - Catalytic domain images:
Western blot - Cathepsin S antibody - Catalytic domain (ab50400)
All lanes : Anti-Cathepsin S antibody - Catalytic domain (ab50400) at 1/1000 dilution
Lane 1 : r-Human Cathepsin S at 0.04 µg
Lane 2 : r-Human Cathepsin S at 0.02 µg
Lane 3 : r-Human Cathepsin S at 0.01 µg
Lane 4 : r-Human Cathepsin S at 0.004 µg
Lane 5 : r-Human Cathepsin S at 0.002 µg
Predicted band size : 37 kDa
The major band is the intact Cathepsin S, and the smaller forms are the single-chain activated Cathepsin S.
Protocols
References for Anti-Cathepsin S antibody - Catalytic domain (ab50400)
ab50400 has not yet been referenced specifically in any publications.
Publishing research using ab50400? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"