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Anti-Cathepsin S antibody - Propeptide domain (ab50399)

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Overview

Product name

Anti-Cathepsin S antibody - Propeptide domain
See all Cathepsin S products (9) ...

Description

Rabbit polyclonal to Cathepsin S - Propeptide domain

Specificity

This antibody reacts with Cathepsin S.

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Immunogen

A synthetic peptide based on the propeptide domain of human Cathepsin S.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Cathepsins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Immunology >> Cell Type Markers >> Non-CD >> APC
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Immunology >> Adaptive Immunity >> MHC >> Class II

  • Western blot - Cathepsin S antibody - Propeptide domain (ab50399)Western blot - Cathepsin S antibody - Propeptide domain (ab50399) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 37 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene.

Cellular localization

Lysosomal.

Alternative names

  • CathepsinS antibody
  • CTSS antibody

Database links

Anti-Cathepsin S antibody - Propeptide domain images:

  Western blot - Cathepsin S antibody - Propeptide domain (ab50399)

Western blot - Cathepsin S antibody - Propeptide domain (ab50399)

All lanes : Anti-Cathepsin S antibody - Propeptide domain (ab50399) at 1/1000 dilution

Lane 1 : Recombinant Human Cathepsin S at 0.04 µg
Lane 2 : Recombinant Human Cathepsin S at 0.02 µg
Lane 3 : Recombinant Human Cathepsin S at 0.01 µg
Lane 4 : Recombinant Human Cathepsin S at 0.004 µg
Lane 5 : Recombinant Human Cathepsin S at 0.002 µg


Predicted band size : 37 kDa

Protocols

  • Recommended WB protocol

    • References for Anti-Cathepsin S antibody - Propeptide domain (ab50399)

    ab50399 has not yet been referenced specifically in any publications.

    Publishing research using ab50399? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"