Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Immunology >> Adaptive Immunity >> MHC >> Class II
Overview
Product name
Anti-Cathepsin S antibody
See all Cathepsin S products (9) ...
Description
Goat polyclonal to Cathepsin S
Specificity
This antibody is specific to Cathepsin S.
Tested applications
IHC-P, IP, WB, IHC-Frmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide mapping to the C terminus of human Cathepsin S. (Peptide available as ab54080.)
Positive control
IHC-P: human lymphnod
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate).
Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Cysteine protease >> Cathepsins
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Immunology >> Cell Type Markers >> Non-CD >> APC
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Lysosome
Immunology >> Adaptive Immunity >> MHC >> Class II
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin S antibody (ab18822)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 20 µg/ml
IP: Use a concentration of 20 µg/ml
WB: Use a concentration of 1 - 4 µg/ml.Detects a band of approximately 30 kDa (predicted molecular weight: 38 kDa).Can be blocked with Cathepsin S peptide (ab54080). (One customer reports that overnight incubation with this antibody at room temperature gave the best staining in Western blot.)
IHC-Fr: Use a concentration of 20 µg/ml
Target
Relevance
Cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. Transcript variants utilizing alternative polyadenylation signals exist for this gene.
Cellular localization
Lysosomal.
Alternative names
- Cathepsin S precursor antibody
- Cathepsin S preproprotein antibody
- CathepsinS antibody
see all
Database links
- Entrez Gene: 1520 Human
- Entrez Gene: 13040 Mouse
- Entrez Gene: 50654 Rat
- Omim: 116845 Human
- SwissProt: P25774 Human
- SwissProt: O70370 Mouse
- SwissProt: Q02765 Rat
- Unigene: 181301 Human
see all
Anti-Cathepsin S antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin S antibody (ab18822)
IHC image of ab18822 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18822, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Cathepsin S antibody (ab18822)
This product has been referenced in:
- Tizon Bet al. Cystatin C protects neuronal cells from amyloid-beta-induced toxicity. J Alzheimers Dis 19:885-94 (2010).Read more (PubMed: 20157244) »
- Ovchinnikova Oet al. T-cell activation leads to reduced collagen maturation in atherosclerotic plaques of Apoe(-/-) mice. Am J Pathol 174:693-700 (2009). IHC-P; Human.Read more (PubMed: 19131590) »
See all 2 publications for this product
Publishing research using ab18822? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"