Anti-Caveolin 1 antibody - Caveolae Marker (ab18199)

thank you very much for your reply. I would like once more to disturb you and ask the following question; quite often you are indicating that antigen was a synthetic peptide. I am working mainly with chick brain and quite often it is impossible to understand if the antibody is reactive with chicks. I am interested if any of your caveolin antibodies (e.g. 93952, 2910 or any other) is reactive against chicken proteins and the same question with antibody 115822 (Anti-eIF2 alpha).

ANSWER:

Thank you very much for your interest in Abcam antibodies.

For most of the antibodies we have in our catalog it is possible to know the immunogen sequence or at least the immunogenic region and compare it with the sequence of interest.

For example, we know that the anti-caveolin ab18199 shows 76% homology with the chicken protein (SwissProt P35431).

If you want to test this antibody for exampleI can offer a discount off a future purchase if you buy ab18199 now, test it inChicken and submit feedback to us in the form of an Abreview.It doesn’t matter whether theAbreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test an Abcam antibody in Chicken. I will then send a discount code. This code must be issued before purchasing the antibody you would like to test, so please wait for my reply before ordering.

2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in in Chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for Chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

If you are interested in this offer, please inform us which application you would like the antibody in so I can narrow down the choice of the antibodies.

I am going to contact the lab where the anti-eIF2 alpha(ab115822) is produced in order to ask for the immunogen sequence or region.

used in IHC-Fr with mouse
sees no difference in staining for WT and KO mice
has Ab been tested for cross-reactivity with caveolin 2 and 3?

ANSWER:

Thank you for contacting us.

Here is the information I have received from our lab:

We haven’t tested this ab for crossreactivity with caveolin 2 and caveolin 3 but I’ve done a quick blast of our immunogen sequence against Mouse and it looks to be specific to caveolin 1.


If it does turn out that the KO mice are fine and it is our antibody, it would be wonderful if youcould let us know. In that case, please provide us with the experimental details and results, so that I can pass it on to our lab.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

May I ask about expiry date of mentioned below antibodies? I couldn't find such info on your website (only storage instruction) and I'm not sure how long can I store them without the loss of their quality. Cat.no. Ab23841 Ab54481 Ab6276 Ab9484 Ab2920 Ab56023 Ab45966 Ab95453 Ab18199 Ab2911 Ab61249

ANSWER:

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

I need to know the immunogen for this product. Description is that it is somewhere within residues 1-100. If you can't reveal the sequence, could I be informed if the sequence completely covers residues 50-100?

ANSWER:

Thank you for your enquiry,

Regarding ab18199 (Caveolin 1 antibody - Caveolae Marker), the immunogenic peptide sequence is not derived from amino acids 50-100.

I hope this information helps. Please don't hesitate to contact us again if you have further questions.