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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-Caveolin 1 antibody - Caveolae Marker
See all Caveolin 1 products (16) ...
Description
Rabbit polyclonal to Caveolin 1 - Caveolae Marker
Tested applications
ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Dog, Human, Pig
Predicted to work with
Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Caveolin 1.
(Peptide available as ab18607.)
Positive control
This antibody gave a positive signal in the following lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Heart (Human) Tissue Lysate Heart (Rat) Tissue Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-P: Use at an assay dependent concentration.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).Can be blocked with Caveolin 1 peptide (ab18607).
Target
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney.
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similarities
Belongs to the caveolin family.
Post-translational
modifications
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Target information above from: UniProt accessionQ03135
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- VIP 21 antibody
- CAV 1 antibody
- CAV antibody
see all
Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- SwissProt: P41350 Rat
see all
Anti-Caveolin 1 antibody - Caveolae Marker images:
Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
ICC/IF image of ab18199 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18199, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
All lanes : Anti-Caveolin 1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Mouse Heart Lysate
Lane 2 : Human Heart Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 20 kDa
Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
All lanes : Anti-Caveolin 1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 20 kDa
Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
ab18199 staining human HT1080 cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubating with ab18199 at 10 µg/ml for 1 hour at 20°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
ICC/IF image of ab18199 stained MEF7cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18199, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Caveolin 1 antibody - Caveolae Marker (ab18199)
This product has been referenced in:
- Resnik Net al. Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes. J Biol Chem 286:1499-507 (2011). WB, ICC/IF; Dog.Read more (PubMed: 21071449) »
- Su Let al. Differential effects of testosterone and TGF-ß3 on endocytic vesicle-mediated protein trafficking events at the blood-testis barrier. Exp Cell Res 316:2945-60 (2010). WB, ICC/IF; Rat.Read more (PubMed: 20682309) »
See all 8 publications for this product
Publishing research using ab18199? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"