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Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
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Product Name
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Caveolin 1 antibody - Caveolae Marker
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See all Caveolin 1 - Subcellular marker antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Caveolin 1 - Caveolae Marker
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Caveolin 1.
(Peptide available as ab18607.)
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Reacts with
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Hu, Ms, Rat, Dog
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Predicted to react
(species key)
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Cow, Pig
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Can be blocked with Caveolin 1 peptide (ab18607).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Heart (Mouse) Tissue Lysate
Heart (Human) Tissue Lysate
Heart (Rat) Tissue Lysate
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Cellular localization
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Cell Membrane
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Relevance
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Caveolin 1 is closely associated with caveloae and are also found outside caveolae. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21 to 24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin 1 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caveolin 1 antibody - Caveolae Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Caveolin 1 antibody - Caveolae Marker images, references, products related to ab18199 and other tools.
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Caveolin 1 antibody - Caveolae Marker images:
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 Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
ICC/IF image of ab18199 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18199, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
All lanes : Caveolin 1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : Mouse Heart Lysate
Lane 2 : Human Heart Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 20 kDa
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab18199)
All lanes : Caveolin 1 antibody - Caveolae Marker (ab18199) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 20 kDa
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Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab18199)
ab18199 staining human HT1080 cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubating with ab18199 at 10 µg/ml for 1 hour at 20°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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References for Caveolin 1 antibody - Caveolae Marker (ab18199)
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This product has been used in: (two most recent references)
Mykoniatis A et al. Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway. Am J Physiol Cell Physiol 298:C85-97 (2010). WB, ICC/IF; Dog. PubMed: 19864322
Agnihothram SS et al. Assembly of arenavirus envelope glycoprotein GPC in detergent-soluble membrane microdomains. J Virol 83:9890-900 (2009). PubMed: 19625404
See all 6 publication references for this product.
If you publish research using ab18199 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Caveolin 1
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Caveolin 1 antibody - Caveolae Marker - more information
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