Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)

Rat astrocytes stained with fluorescently labelled Caveolin antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).

This image was kindly supplied as part of the review submitted by Donghui Zhu.

Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)

All lanes : Anti-Caveolin 1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml

Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen

Lysates/proteins at 20 µg per lane.

Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution

Predicted band size : 20 kDa
Observed band size : 20 kDa

Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)

ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody. Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.

This image is courtesy of an Abreview by William Ackerman.

Immunoprecipitation - Caveolin 1 antibody - Caveolae Marker (ab2910)

ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation.  400 µg of lysate was present in the input.

An HRP-conjugated goat anti-rabbit was used for the Western Blot step.

Lane 1: Whole Cell

Lane 2: IP-cav-1

Lane 3: Unrelated antibody

Lane 4: Unrelated antibody

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin 1 antibody - Caveolae Marker (ab2910)

ab2910 staining Caveolin 1 - Caveolae Marker in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37ºC. A Cy3^®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)

Predicted band size : 20 kDa


Western blot of caveolin-1 on rat heart protein extract using ab2910.