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Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)
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Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
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Product Name
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Caveolin 1 antibody - Caveolae Marker
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See all Caveolin 1 - Subcellular marker antibodies (18)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Caveolin 1 - Caveolae Marker
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Immunogen
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Synthetic Peptide: M(1)SGGKYVDSEGHLYTVP(17)C.
Immunizing peptide corresponds to amino acid residues 1-17 from human caveolin-1.
This sequence is completely conserved between human, mouse, canine, bovine, and rat.
BLAST 'MSGGKYVDSEGHLYTVP-C' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat, ChHm, Dog, Hm
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Specificity
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Detects caveolin-1.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/500
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 20 kDa. This antibody detects an ~22 kDa protein representing caveolin-1 from rat heart protein extract.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human lung, heart or spleen
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Cellular localization
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Cell Membrane
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Relevance
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Caveolin 1 is closely associated with caveloae and are also found outside caveolae. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21 to 24 kDa integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin 1 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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PBS, 1 mg/ml BSA, 0.05% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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This antibody can be used as a marker for lipid raft fractions.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Caveolin 1 antibody - Caveolae Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Caveolin 1 antibody - Caveolae Marker images, references, products related to ab2910 and other tools.
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Caveolin 1 antibody - Caveolae Marker images:
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 Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
Rat astrocytes stained with fluorescently labelled Caveolin antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).
This image was kindly supplied as part of the review submitted by Donghui Zhu.
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)
All lanes : Caveolin 1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml
Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
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Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody. Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image. This image is courtesy of an Abreview by William Ackerman.
See Abreview
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Immunoprecipitation - Caveolin 1 antibody - Caveolae Marker (ab2910)
ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation. 400 µg of lysate was present in the input. An HRP-conjugated goat anti-rabbit was used for the Western Blot step. Lane 1: Whole Cell Lane 2: IP-cav-1 Lane 3: Unrelated antibody Lane 4: Unrelated antibody This image is courtesy of an anonymous Abreview
See Abreview
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Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)
Predicted band size : 20 kDa
Western blot of caveolin-1 on rat heart protein extract using ab2910.
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References for Caveolin 1 antibody - Caveolae Marker (ab2910)
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This product has been used in: (two most recent references)
Bento-Abreu A et al. Albumin endocytosis via megalin in astrocytes is caveola- and Dab-1 dependent and is required for the synthesis of the neurotrophic factor oleic acid. J Neurochem 111:49-60 (2009). ICC/IF; Rat. PubMed: 19656258
Bhattacharya B & Roy P Bluetongue virus outer capsid protein VP5 interacts with membrane lipid rafts via a SNARE domain. J Virol : (2008). PubMed: 18753209
See all 10 publication references for this product.
If you publish research using ab2910 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Caveolin 1
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