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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-Caveolin 1 antibody - Caveolae Marker
See all Caveolin 1 products (16) ...
Description
Rabbit polyclonal to Caveolin 1 - Caveolae Marker
Specificity
Detects caveolin-1.
Tested applications
ICC/IF, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Hamster, Dog, Human, Chinese Hamster
Immunogen
Synthetic Peptide: M(1)SGGKYVDSEGHLYTVP(17)C. Immunizing peptide corresponds to amino acid residues 1-17 from human caveolin-1. This sequence is completely conserved between human, mouse, canine, bovine, and rat.
MSGGKYVDSE GHLYTVP-C
Positive control
Human lung, heart or spleen
General notes
This antibody can be used as a marker for lipid raft fractions.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500
IHC-Fr: Use at an assay dependent dilution. (PubMed: 20351069)
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
Target
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney.
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similarities
Belongs to the caveolin family.
Post-translational
modifications
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Target information above from: UniProt accessionQ03135
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- VIP 21 antibody
- CAV 1 antibody
- CAV antibody
see all
Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- SwissProt: P41350 Rat
- Unigene: 74034 Human
see all
Anti-Caveolin 1 antibody - Caveolae Marker images:
Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
Rat astrocytes stained with fluorescently labelled Caveolin antibody. Primary antibody is ab2910 (dilution 1/500) and the secondary antibody is Texas red labelled anti-rabbit IgG (dilution1/1000).
This image was kindly supplied as part of the review submitted by Donghui Zhu.
Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)
All lanes : Anti-Caveolin 1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml
Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
Immunocytochemistry/ Immunofluorescence - Caveolin 1 antibody - Caveolae Marker (ab2910)
ab2910 at 1/250 dilution staining mouse NIH 3T3 cells by Immunocytochemistry (panel B). The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody. Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.
This image is courtesy of an Abreview by William Ackerman.
Immunoprecipitation - Caveolin 1 antibody - Caveolae Marker (ab2910)
ab2910 diluted 1/500 and was incubated with A549 whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation. 400 µg of lysate was present in the input.
An HRP-conjugated goat anti-rabbit was used for the Western Blot step.
Lane 1: Whole Cell
Lane 2: IP-cav-1
Lane 3: Unrelated antibody
Lane 4: Unrelated antibody
This image is courtesy of an anonymous Abreview
Western blot - Caveolin 1 antibody - Caveolae Marker (ab2910)
Predicted band size : 20 kDa
Western blot of caveolin-1 on rat heart protein extract using ab2910.
References for Anti-Caveolin 1 antibody - Caveolae Marker (ab2910)
This product has been referenced in:
- Fernandes Ret al. Reactive oxygen species downregulate glucose transport system in retinal endothelial cells. Am J Physiol Cell Physiol 300:C927-36 (2011).Read more (PubMed: 21228321) »
- Sabra G & Vermette P Endothelial cell responses towards low-fouling surfaces bearing RGD in a three-dimensional environment. Exp Cell Res 317:1994-2006 (2011).Read more (PubMed: 21679704) »
See all 16 publications for this product
Publishing research using ab2910? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"