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- Secondary antibodies
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Product name
Anti-Caveolin 1 antibody
See all Caveolin 1 products (19) ...
Description
Rabbit polyclonal to Caveolin 1
Specificity
This antibody detects endogenous levels of total Caveolin 1 protein.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
The antiserum was produced against synthesized non phosphopeptide derived from human Caveolin 1 around the phosphorylation site of Tyrosine 14.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS(without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Cholesterol Metabolism
Cancer >> Cancer Metabolism >> Response to hypoxia
Cancer >> Tumor biomarkers >> Other
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Caveolae and Clathrin
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Coat Proteins
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Predicted molecular weight: 20 kDa.
ELISA
ELISA: 1/20000.ELISA: 1/20000.
IHC-P: Use a concentration of 1 µg/ml.
Target
Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney.
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similarities
Belongs to the caveolin family.
Post-translational
modifications
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Target information above from: UniProt accessionQ03135
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- VIP 21 antibody
- CAV 1 antibody
- CAV antibody
see all
Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- SwissProt: P41350 Rat
- Unigene: 74034 Human
see all
Anti-Caveolin 1 antibody images:
Western blot - Caveolin 1 antibody (ab39541)
Predicted band size : 20 kDa
Western blot analysis of the extracts from NIH/3T3 cells untreated or treated with H2O2 using Caveolin 1 antibod (ab39541) and Caveolin 1(Phospho-Tyr14) antibody.
Lane 1: Using ab39541;
Lane 2: Using ab39541 preincubated with synthesized peptide;
Lane 3: Using Caveolin 1(Phospho-Tyr14) antibody;
Lane 4: Using Caveolin 1(Phospho-Tyr14) antibody preincubated with synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Caveolin 1 antibody(ab39541)
ab39541 staining caveolin in human lung.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Caveolin 1 antibody (ab39541)
ab39541 has not yet been referenced specifically in any publications.
Publishing research using ab39541? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"