Overview

  • Product nameAnti-Cbl-c antibody
    See all Cbl-c primary antibodies
  • Description
    Rabbit polyclonal to Cbl-c
  • SpecificityNo reactivity is expected with Cbl-a or Cbl-b.
  • Tested applicationsELISA, WB, IP, ICC/IFmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human, Chimpanzee
  • Immunogen

    Synthetic peptide:

    PRKPRNAQPKVRLLK

    , corresponding to amino acids 444-458 of Human Cbl-c

  • Positive control
    • Hek 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab34750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/50000.
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceCblc belongs to a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cblc is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitous). Cblc, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cblc knock out mice show no obvious phenotype. Thus, the physiological role of Cblc is not known.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Cas Br M (murine) ecotropic retroviral transforming sequence c antibody
    • Cas Br M (murine) ectropic retroviral transforming sequence c antibody
    • Casitas B lineage lymphoma c antibody
    • CBL 3 antibody
    • CBL SL antibody
    • RING finger protein 57 antibody
    • RNF57 antibody
    • SH3 binding protein CBL C antibody
    • Signal transduction protein CBL C antibody
    see all

Anti-Cbl-c antibody images

  • All lanes : Anti-Cbl-c antibody (ab34750) at 1/500 dilution

    Lane 1 : Hek 293T cell lysate transfected with empty vector
    Lane 2 : Hek 293T cell lysate transfected with Cbl-c.
    Lane 3 : Hek 293T cell lysate transfected with empty vector
    Lane 4 : Hek 293T cell lysate transfected with Cbl-c.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Image courtesy of Human Protein Atlas
    ab34750 (1/500) showing strong nuclear and cytoplasmic stainning of cells of the kidney tubuli. Paraffin embedded human kidney tissue was incubated with ab123 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

References for Anti-Cbl-c antibody (ab34750)

ab34750 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (7.5)
Sample Mouse Tissue lysate - whole (Intestine Brain mammary gland)
Specification Intestine Brain mammary gland
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Neha

Verified customer

Submitted Jan 24 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"