Anti-Cbl-c antibody (ab34750)

Overview

• Product nameAnti-Cbl-c antibodySee all Cbl-c primary antibodies ...
• Description
  Rabbit polyclonal to Cbl-c
• SpecificityNo reactivity is expected with Cbl-a or Cbl-b.
• Tested applicationsELISA, WB, IP, ICC/IF more details
• Species reactivity
  
  Predicted to work with: Mouse, Rat, Human, Chimpanzee
• Immunogen

  Synthetic peptide: PRKPRNAQPKVRLLK, corresponding to amino acids 444-458 of Human Cbl-c

  Run BLAST with Run BLAST with
• Positive controlHek 293T cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe product was affinity purified from monospecific antiserum by immunoaffinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Other

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Ubiquitin ligases

Applications

Target

• RelevanceCblc belongs to a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cblc is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitous). Cblc, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cblc knock out mice show no obvious phenotype. Thus, the physiological role of Cblc is not known.
• Cellular localizationNuclear
• Database links
  • Entrez Gene: 23624 Human
  • Entrez Gene: 23624 Human
  • Entrez Gene: 80794 Mouse
  • Entrez Gene: 292699 Rat
  • Omim: 608453 Human
  • SwissProt: Q9ULV8 Human
  • SwissProt: Q9ULV8 Human
  • SwissProt: Q80XL1 Mouse

  • Unigene: 466907 Human
  • Unigene: 20550 Rat

  see all

• Alternative names
  Cas Br M (murine) ecotropic retroviral transforming sequence c antibodyCas Br M (murine) ectropic retroviral transforming sequence c antibodyCasitas B lineage lymphoma c antibody

  CBL 3 antibodyCBL SL antibodyRING finger protein 57 antibodyRNF57 antibodySH3 binding protein CBL C antibodySignal transduction protein CBL C antibody

  see all

Anti-Cbl-c antibody images

• 

  Western blot - Cbl-c antibody (ab34750)
  All lanes : Anti-Cbl-c antibody (ab34750) at 1/500 dilution
  
  Lane 1 : Hek 293T cell lysate transfected with empty vector
  Lane 2 : Hek 293T cell lysate transfected with Cbl-c.
  Lane 3 : Hek 293T cell lysate transfected with empty vector
  Lane 4 : Hek 293T cell lysate transfected with Cbl-c.
  
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cbl-c antibody (ab34750)
  Image courtesy of Human Protein Atlas
  
  ab34750 (1/500) showing strong nuclear and cytoplasmic stainning of cells of the kidney tubuli. Paraffin embedded human kidney tissue was incubated with ab123 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.