Overview

  • Product nameAnti-Cdc14A antibody [DCS-291]
    See all Cdc14A primary antibodies
  • Description
    Mouse monoclonal [DCS-291] to Cdc14A
  • Tested applicationsWB, ICC, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human).

  • Positive control
    • A431 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab10536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 67 kDa.
ICC Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

Target

  • RelevanceOverproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53.
  • Cellular localizationCytoplasmic, Cytoskeleton, Nuclear.
  • Database links
  • Alternative names
    • CDC 10 antibody
    • Cdc 14 antibody
    • Cdc 14A antibody
    • Cdc 14A1 antibody
    • Cdc 14A2 antibody
    • CDC10 (cell division cycle 10, S. cerevisiae, homolog) antibody
    • CDC10 antibody
    • Cdc14 antibody
    • CDC14 cell division cycle 14 homolog A antibody
    • CDC14 homolog A antibody
    • Cdc14A1 antibody
    • Cdc14A2 antibody
    • Cell Division Cycle 14 Homolog A antibody
    • Cell division cycle 14, S. cerevisiae, homolog A antibody
    • Cell division cycle 14A antibody
    • Dual specificity protein phosphatase CDC 14A antibody
    • Dual specificity protein phosphatase CDC14A antibody
    • hCDC 14 antibody
    • hCDC14 antibody
    • OTTHUMP00000012653 antibody
    • OTTHUMP00000012654 antibody
    • OTTHUMP00000012655 antibody
    see all

References for Anti-Cdc14A antibody [DCS-291] (ab10536)

This product has been referenced in:
  • Farhana L  et al. Upregulation of miR-150* and miR-630 induces apoptosis in pancreatic cancer cells by targeting IGF-1R. PLoS One 8:e61015 (2013). WB ; Human . Read more (PubMed: 23675407) »

See 1 Publication for this product

Product Wall

Thank you very much for your kind words and also for contacting me to let me know you had success. Can you please submit a review? You can submit a review by clicking on the "reviewing" link on the datasheet. You will receive Abpoints for doing this...

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Thank you for your enquiry. Ab10536 has been tested for application in Immunocytochemistry but unfortunately, we do not have more information. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconv...

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Thank you for getting back to us and providing more details of your results. Your feedback is very important us. Since you have some results, we can offer you a new vial free of charge. Certainly, we will take your comment regarding the unit size v...

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Thank you for your patience. We have contacted the originator to get further information about the exact sequence of the immunogen. We regret to inform you that unfortunately we are unable to provide this information for you. If you are dissatisfie...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"